The murine cytomegalovirus immunomodulatory gene m152 prevents recognition of infected cells by M45-specific CTL but does not alter the immunodominance of the M45-specific CD8 T cell response in vivo
The murine cytomegalovirus immunomodulatory gene m152 prevents recognition of infected cells by M45-specific CTL but does not alter the immunodominance of the M45-specific CD8 T cell response in vivo
复制标题
DOI：
10.4049/jimmunol.169.1.359
复制
发表时间：
2002-07-01
影响因子：
4.4
通讯作者：
Fling, SP
中科院分区：
文献类型：
--
作者：
Gold, MC;Munks, MW;Fling, SP
尽管体外研究表明疱疹病毒，包括鼠CMV（MCMV），编码干扰MHC I类途径的基因，但其对体内CTL应答的影响尚不清楚。我们通过使用从缺乏m152的MCMV感染小鼠中分离的CTL克隆筛选MCMV基因组文库，从MCMV M45中鉴定了D-b限制性CTL表位。由于m152在体外严重抑制CTL对M45的识别，我们质疑在感染表达m152的野生型MCMV的小鼠中是否会产生M45特异性应答。用野生型MCMV或MCMVDeltam 152感染的小鼠对M45 Ag产生类似的反应。此外，我们没有看到在用m152感染MCMV后，总MCMV特异性应答中M45特异性CD 8 T细胞的比例发生偏移。尽管m152在体外对M45的呈递具有深远的影响，但它不影响M45在体内CTL应答中的免疫优势。
Although in vitro studies have shown that herpesviruses, including murine CMV (MCMV), encode genes that interfere with the MHC class I pathway, their effects on the CTL response in vivo is unclear. We identified a D-b-restricted CTL epitope from MCMV M45 by screening an MCMV genomic library using CTL clones isolated from mice infected with MCMV lacking m152. Because m152 severely inhibits CTL recognition of M45 in vitro, we questioned whether an M45-specific response would be generated in mice infected with wild-type MCMV expressing m152. Mice infected with wild-type MCMV or MCMVDeltam152 made similar responses to the M45 Ag. Moreover, we saw no skewing of the proportion of M45-specific CD8 T cells within the total MCMV-specific response after infection with MCMV with m152. Despite the profound effect m152 has on presentation of M45 in vitro, it does not affect the immunodominance of M45 in the CTL response in vivo.