Intensification of mass transfer in aqueous two‐phase systems
Intensification of mass transfer in aqueous two‐phase systems
复制标题
水性两相体系中传质的强化
DOI：
10.1002/bit.260410110
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发表时间：
1993
影响因子：
3.8
通讯作者：
S. V. Kumar
中科院分区：
文献类型：
--
作者：
S. Save;V. G. Pangarkar;S. V. Kumar
开发了一种新技术，该技术通过将分散相转化为胶体气体泡沫（CGA）来强化传统的双水相萃取，用于提取酶。在本工作中，使用聚乙二醇-硫酸钠-水系统提取淀粉葡糖苷酶（1，4-α-D-葡聚糖葡糖水解酶）。较轻的相，即，将富含聚乙二醇（PEG）的相转化成CGA，然后将CGA分散到富含盐的相中。考察了表面活性剂种类及其浓度、分散相流速、相组成和分布器类型对分散相传质系数的影响。结果表明，与喷雾塔相比，传质系数值高9-16倍。在浓度为0.33 g/L的十六烷基三甲基氯化铵的多孔分布器产生最好的结果。John Wiley & Sons，Inc.
A novel technique which intensifies conventional aqueous two‐phase extraction by conversion of dispersed phase into colloidal gas aphrons (CGAs) has been developed for extraction of an enzyme. In the present work, amyloglucosidase (1,4‐α‐D‐glucan glucohydrolase) was extracted using a polyethylene glycol‐sodium sulfate‐water system. The lighter phase, i.e., polyethylene glycol (PEG) rich phase, was converted into CGAs which were then dispersed into a salt rich phase. The effect of type of surfactant and its concentration, dispersed phase velocity, phase composition, and type of sparger on the dispersed phase mass transfer coefficient was investigated. The results suggests 9–16 times higher values of mass transfer coefficient compared to spray column. The multiorifice sparger at concentrations of 0.33 g/L of cetyl trimethyl ammonium chloride yielded best results. © 1993 John Wiley & Sons, Inc.