A fluorescent glucose bioassay based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters
A fluorescent glucose bioassay based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters
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基于过氧化氢诱导的由 MnO2 纳米片和铜纳米团簇组成的淬灭系统分解的荧光葡萄糖生物测定
DOI：
10.1007/s00604-016-2045-7
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发表时间：
2017-02-01
影响因子：
5.7
通讯作者：
Liu, Yan-Ming
中科院分区：
文献类型：
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作者：
Wang, Hai-Bo;Chen, Ying;Liu, Yan-Ming
摘要作者描述了一种基于使用二氧化锰纳米片和铜纳米团簇（CuNC）作为纳米探针的荧光葡萄糖测定法。以牛血清白蛋白为模板，通过硫酸铜（II）的化学还原合成了CuNC。在将MnO 2纳米片添加到CuNC的胶体溶液中时，CuNC的荧光（在335/410 nm的激发/发射波长下测量）被淬灭。然而，在酶促产生的H2 O2的存在下，MnO 2纳米片被还原以形成Mn（II）离子。结果，荧光强度恢复。葡萄糖含量测定基于葡萄糖氧化酶对葡萄糖的酶促转化，生成H2 O2和葡萄糖醛酸。校准曲线在1 μM至200 μM葡萄糖浓度范围内呈线性，检测限为100 nM。该方法已成功地应用于加标人血清样品中葡萄糖的测定。
图形摘要一个灵敏的荧光生物测定法的基础上过氧化氢诱导的猝灭剂系统的分解MnO 2纳米片和铜纳米团簇（CuNC）的葡萄糖检测报告。
AbstractThe authors describe a fluorometric glucose assay that is based on the use of MnO2 nanosheets and copper nanoclusters (CuNCs) acting as nanoprobes. The CuNCs were synthesized by using bovine serum albumin as a template by chemical reduction of copper(II) sulfate. On addition of MnO2 nanosheets to a colloidal solution of CuNCs, the fluorescence of CuNCs (measured at excitation/emission wavelengths of 335/410 nm) is quenched. However, in the presence of enzymatically generated H2O2, the MnO2 nanosheets are reduced to form Mn(II) ions. As a result, fluorescence intensity recovers. The glucose assay is based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2 and glucuronic acid. The calibration plot is linear in the 1 μM to 200 μM glucose concentration range, and the detection limit is 100 nM. The method was successfully applied to the determination of glucose in spiked human serum samples.
Graphical abstractA sensitive fluorescent bioassay is reported for the detection of glucose based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters (CuNCs).