Paraventricular Nucleus Infusion of Epigallocatechin-3-O-Gallate Improves Renovascular Hypertension
Paraventricular Nucleus Infusion of Epigallocatechin-3-O-Gallate Improves Renovascular Hypertension
复制标题
室旁核输注表没食子儿茶素-3-O-没食子酸酯可改善肾血管性高血压
DOI：
10.1007/s12012-015-9335-x
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发表时间：
2016
影响因子：
3.2
通讯作者：
Yu-Ming Kang
中科院分区：
文献类型：
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作者：
Qiu-Yue Yi;Jie Qi;Xiao-Jing Yu;Hong-Bao Li;Yan Zhang;Qing Su;Tao Shi;Dong-Mei Zhang;Jing Guo;Zhi-Peng Feng;Mo-Lin Wang;Guo-Qing Zhu;Jin-Jun Liu;Xiao-Lian Shi;Yu-Ming Kang
氧化应激在高血压的发病机制中起重要作用。表没食子儿茶素-3-O-没食子酸酯（EGCG）是绿色茶中存在的主要多酚，以其有效的抗氧化和抗炎特性而闻名。在本研究中，我们假设，EGCG减弱氧化应激下丘脑室旁核（PVN），从而降低血压和交感神经活性在肾血管性高血压大鼠。雄性Sprague-Dawley大鼠采用两肾一夹（2K-1C）法建立肾血管性高血压模型后，通过渗透压微泵向双侧PVN灌注EGCG（20 μg/h）或溶媒4周。结果表明：（1）2K-1C引起的高血压与室旁核活性氧的产生有关;（2）慢性注射EGCG可降低应激相关的NAD（P）H氧化酶亚基gp 91 phox和NOX-4的含量，提高抗氧化酶SOD-1的活性，平衡细胞因子的含量（2）IL-1β、IL-6、IL-10和MCP-1在PVN中的表达，降低血浆去甲肾上腺素水平。我们的研究结果提供了强有力的证据表明，通过在PVN中的有效抗氧化和抗炎活性，EGCG的PVN输注抑制了肾血管性高血压的进展。
Oxidative stress plays an important role in the pathogenesis of hypertension. Epigallocatechin-3-O-gallate (EGCG) is the main polyphenol present in green tea and is known for its potent antioxidant and anti-inflammatory properties. In the present study, we hypothesize that EGCG attenuates oxidative stress in the paraventricular nucleus of hypothalamus (PVN), thereby decreasing the blood pressure and sympathetic activity in renovascular hypertensive rats. After renovascular hypertension was induced in male Sprague-Dawley rats by the two-kidney one-clip (2K-1C) method, the rats received bilateral PVN infusion of EGCG (20 μg/h) or vehicle via osmotic minipump for 4 weeks. Our results were shown as follows: (1) Hypertension induced by 2K-1C was associated with the production of reactive oxygen species in the PVN; (2) chronic infusion of EGCG in the PVN decreased stress-related NAD(P)H oxidase subunit gp91phoxand NOX-4 and increased the activity of antioxidant enzymes (SOD-1), also balanced the content of cytokines (IL-1β, IL-6, IL-10 and MCP-1) in the PVN, and attenuated the level of norepinephrine in plasma of 2K-1C rats. Our findings provide strong evidence that PVN infusion of EGCG inhibited renovascular hypertension progression through its potent anti-oxidative and anti-inflammatory activity in the PVN.