The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules.
The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules.
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DOI：
10.1038/ncomms11266
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发表时间：
2016-04-13
影响因子：
16.6
通讯作者：
Dow JA
中科院分区：
文献类型：
--
作者：
Halberg KA;Rainey SM;Veland IR;Neuert H;Dornan AJ;Klämbt C;Davies SA;Dow JA
多细胞生物依靠细胞粘附分子来协调细胞间的相互作用，并在组织形成过程中提供导航线索。在果蝇中，Fasciclin 2（Fas 2）由于其在神经系统发育和维持中的作用而被深入研究;然而，Fas 2在成年肾（Malpighian）小管中而不是在神经元组织中最丰富地表达。Fas 2在该上皮中的作用尚不清楚。在这里，我们表明，Fas 2是必不可少的刷状缘维持肾小管的果蝇。Fas 2在肾小管形态发生期间动态表达，每当组织具有运输能力时定位于刷状缘。Fas 2表达水平的遗传操作影响微绒毛长度和组织，这反过来又显著影响组织的液体分泌的刺激速率。因此，我们证明了一个根本不同的作用，这个众所周知的细胞粘附分子，并建议Fas 2介导的微绒毛间的嗜同性粘附复合物有助于稳定刷状缘。 在果蝇中，Fasciclin 2（Fas 2）主要在神经系统中进行研究，但这种粘附蛋白在成年肾小管中更丰富。在这里，作者表明，Fas 2通过调节微绒毛长度和组织对肾小管刷状缘的维持至关重要。
Multicellular organisms rely on cell adhesion molecules to coordinate cell–cell interactions, and to provide navigational cues during tissue formation. In Drosophila, Fasciclin 2 (Fas2) has been intensively studied due to its role in nervous system development and maintenance; yet, Fas2 is most abundantly expressed in the adult renal (Malpighian) tubule rather than in neuronal tissues. The role Fas2 serves in this epithelium is unknown. Here we show that Fas2 is essential to brush border maintenance in renal tubules of Drosophila. Fas2 is dynamically expressed during tubule morphogenesis, localizing to the brush border whenever the tissue is transport competent. Genetic manipulations of Fas2 expression levels impact on both microvilli length and organization, which in turn dramatically affect stimulated rates of fluid secretion by the tissue. Consequently, we demonstrate a radically different role for this well-known cell adhesion molecule, and propose that Fas2-mediated intermicrovillar homophilic adhesion complexes help stabilize the brush border. In Drosophila, Fasciclin 2 (Fas2) has been mainly studied in the nervous system, yet this adhesion protein is more abundant in the adult renal tubule. Here the authors show that Fas2 is essential for brush border maintenance in renal tubules through regulation of microvilli length and organization.