Fabrication of strontium-incorporated protein supramolecular nanofilm on titanium substrates for promoting osteogenesis
Fabrication of strontium-incorporated protein supramolecular nanofilm on titanium substrates for promoting osteogenesis
复制标题
钛基底上掺锶蛋白超分子纳米膜的制备促进成骨
DOI：
10.1016/j.msec.2020.110851
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发表时间：
2020-06-01
影响因子：
7.9
通讯作者：
Liu, Ruirui
中科院分区：
文献类型：
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作者：
Ding, Yao;Yuan, Zhang;Liu, Ruirui
钛基质固有的生物惰性是骨科和牙科临床应用中的一个普遍挑战。本研究采用超分子自组装方法在钛表面制备了含锶相变溶菌酶涂层，以促进钛的骨整合。通过扫描电子显微镜(SEM)、原子力显微镜(AFM)、X射线光电子能谱(XPS)和接触角(CA)测试，证明在钛基材上成功地制备了掺锶溶菌酶纳米膜(Ti-Ly-Sr)。细胞形态观察、细胞活力测定、碱性磷酸酶染色及定量分析结果表明，Ti-Ly-Sr基质可促进骨髓基质细胞的早期黏附、增殖和成骨分化。实时定量聚合酶链式反应(qRT-PCR)检测证实，Ti-Ly-sr在分子水平上促进了BMSCs成骨相关基因BMP2、OPG、Runx2和Col-1的表达。此外，Micro-CT和组织学分析证明，掺锶溶菌酶纳米膜修饰的钛种植体在种植4周后具有明显的体内新骨形成能力。我们预计，本系统将为钛基种植体的表面改性提供一种简便而有效的手段。本研究的相关思路和技术将有助于新型植入装置的开发。
The inherent biological inertness of Ti substrates is a general challenge in orthopedic and dental clinical application. In this study, the strontium-containing phase change lysozyme coating was prepared on titanium via a supramolecular self-assembly method for accelerating osseointegration. Successful preparation of the Sr-incorporated lysozyme nanofilm onto the Ti substrate (Ti-Ly-Sr) was proved by tests of scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and Contact angle (CA). Furthermore, the results of cell morphology observation, cell viability assay, alkaline phosphatase staining and quantitative analysis showed that Ti-Ly-Sr substrate enhanced early adhesion, proliferation and osteogenic differentiation of bone marrow stromal cells (BMSCs). The quantitative real-time polymerase chain reaction (qRT-PCR) assays confirmed that Ti-Ly-Sr enhanced the expression of osteogenic related genes (BMP2, OPG, Runx2 and COL-1) of BMSCs at the molecular level. Moreover, Micro-CT and histological analysis proved that the strontium-incorporated lysozyme nanofilm modified Ti implants had significant capability of new bone formation in vivo after implantation for 4 weeks. We anticipate that the present system would provide a facile and effective means for surface modification of Ti-based implants. Relevant ideas and techniques in this study will contribute to the development of new implant devices.