Chitosan nanoparticles encapsulated vesicular systems for oral immunization: preparation, in-vitro and in-vivo characterization
Chitosan nanoparticles encapsulated vesicular systems for oral immunization: preparation, in-vitro and in-vivo characterization
复制标题
DOI：
10.1211/jpp.58.3.0003
复制
发表时间：
2006-03-01
影响因子：
3.3
通讯作者：
Vyas, SP
中科院分区：
文献类型：
--
作者：
Jain, S;Sharma, RK;Vyas, SP
制备了负载BSA的壳聚糖纳米粒，并将其封装在囊泡（脂质体和囊泡体）中，以使其在口服给药时具有耐酸性。制备的系统进行了表征体外的形状，大小，包封率和在模拟胃液（SGF，pH 1.2）和模拟肠液（SIF，pH 7.5）中的稳定性。通过在白化病大鼠中口服施用各种制剂后测量血清IgG滴度和粘膜分泌物中的分泌型伊加（sIgA）水平来研究免疫刺激活性。与未修饰的壳聚糖纳米颗粒相比，口服新型纳米颗粒囊泡制剂后获得了显著更高（P < 0.05）的血清IgG滴度。此外，粘膜分泌物中的高sIgA水平主张囊泡中封装的壳聚糖纳米颗粒作为口服疫苗递送载体-佐剂系统的可能应用。
BSA-loaded chitosan nanoparticles were prepared and encapsulated in vesicles (liposomes and niosomes) to make them acid resistant upon oral administration. Prepared systems were characterized invitro for shape, size, entrapment efficiency and stability in simulated gastric fluid (SGF, pH 1.2) and simulated intestinal fluid (SIF, pH 7.5). The immune stimulating activity was studied by measuring serum IgG titre and secretory IgA (sIgA) levels in mucosal secretions following oral administration of various formulations in albino rats. Significantly higher (P < 0.05) serum IgG titres were achieved following oral administration of novel nanoparticulate vesicular formulations as compared with unmodified chitosan nanoparticles. Further, high sIgA levels in mucosal secretions advocated a possible application of chitosan nanoparticle encapsulated in vesicles as an oral vaccine delivery carrier-adjuvant system.