In vivo two-photon voltage-sensitive dye imaging reveals top-down control of cortical layers 1 and 2 during wakefulness
In vivo two-photon voltage-sensitive dye imaging reveals top-down control of cortical layers 1 and 2 during wakefulness
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DOI：
10.1073/pnas.0802462105
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发表时间：
2008-05-27
影响因子：
11.1
通讯作者：
Bruno, R. M.
中科院分区：
文献类型：
--
作者：
Kuhn, B.;Denk, W.;Bruno, R. M.
传统的成像膜电位变化的方法具有有限的空间分辨率，特别是垂直于皮质表面的长轴。然而，皮层的层状组织表明，活动在深度上的分布是不均匀的。我们开发了一种技术，以解决网络活动的不同皮层在体内使用双光子显微镜的电压敏感染料（VSD）ANNINE-6。我们对头部受限小鼠躯体感觉皮层桶场的自发电压变化进行了成像，并分析了它们在麻醉和清醒期间的时空相关性。EEG记录总是更强的相关性与VSD信号在层（L）2比在L1。附近（
Conventional methods of imaging membrane potential changes have limited spatial resolution, particularly a long the axis perpendicular to the cortical surface. The laminar organization of the cortex suggests, however, that the distribution of activity in depth is not uniform. We developed a technique to resolve network activity of different cortical layers in vivo using two-photon microscopy of the voltage-sensitive dye (VSD) ANNINE-6. We imaged spontaneous voltage changes in the barrel field of the somatosensory cortex of head-restrained mice and analyzed their spatio-temporal correlations during anesthesia and wakefulness. EEG recordings always correlated more strongly with VSD signals in layer (L) 2 than in L1. Nearby (