Effects of administration of cholecystokinin into the VTA on DA overflow in nucleus accumbens and amygdala of freely moving rats.
Effects of administration of cholecystokinin into the VTA on DA overflow in nucleus accumbens and amygdala of freely moving rats.
复制标题
将胆囊收缩素注入 VTA 对自由活动大鼠伏核和杏仁核 DA 溢出的影响。
DOI：
10.1016/0006-8993(95)00518-u
复制
发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Freeman,AS
中科院分区：
文献类型：
--
作者：
Hamilton,ME;Freeman,AS
胆囊收缩素（CCK-8）的羧基端八肽与多巴胺（DA）共存于腹侧被盖区（VTA）的中边缘神经元中。在本研究中，使用自由活动大鼠的体内微透析来评估硫酸化CCK-8（CCK-8S）、未硫酸化CCK-8（CCK-8US）和CCK四肽（CCK-4）局部注射到VTA中对两个中脑边缘DA/CCK-8S末端区域、伏核和杏仁核的DA溢出的相对影响。与电生理学结果一致，显微注射 CCK-8S（而非 CCK-8US 或 CCK-4）引起两个末端区域 DA 溢出的增加。在缺乏 VTA 区域中含有 CCK 的纤维的解剖学证据的情况下，似乎可以合理地得出这样的结论：CCK-8S 通过体细胞树突区域的作用对末端 DA 溢出的调节代表了这些细胞的一种自我调节形式。 CCK-8US 和 CCK-4 是优先的 CCK-B 受体激动剂，而 CCK-8S 非选择性结合 CCK-A 和 CCK-B 受体。因此，这些结果表明 CCK-A 受体参与了 CCK-8S 对 VTA DA 神经元的刺激作用。
The carboxyterminal octapeptide of cholecystokinin (CCK-8) coexists with dopamine (DA) in mesolimbic neurons of the ventral tegmental area (VTA). In the present study, in vivo microdialysis in freely moving rats was used to assess the relative effects of sulfated CCK-8 (CCK-8S), unsulfated CCK-8 (CCK-8US) and CCK tetrapeptide (CCK-4), focally injected into the VTA, on DA overflow in two mesolimbic DA/CCK-8S terminal regions, the nucleus accumbens and the amygdala. Consistent with electrophysiological findings, microinjection of CCK-8S, but not CCK-8US or CCK-4, elicited increases in DA overflow in both terminal regions. In the absence of anatomical evidence of CCK-containing fibers in the VTA region, it seems reasonable to conclude that the modulation of terminal DA overflow by CCK-8S through actions at the somatodendritic region represents a form of autoregulation of these cells. Whereas CCK-8US and CCK-4 are preferential CCK-B receptor agonists, CCK-8S binds non-selectively to CCK-A and CCK-B receptors. Thus, these results implicate CCK-A receptors in the stimulatory effects of CCK-8S on VTA DA neurons.