Sohlh1 is essential for spermatogonial differentiation
Sohlh1 is essential for spermatogonial differentiation
复制标题
DOI：
10.1016/j.ydbio.2006.02.027
复制
发表时间：
2006-06-01
影响因子：
2.7
通讯作者：
Rajkovic, A.
中科院分区：
文献类型：
--
作者：
Ballow, D.;Meistrich, M. L.;Rajkovic, A.
精原细胞是一种既能自我更新又能分化为精母细胞的成体生殖系干细胞。对精原细胞分化所必需的因素知之甚少。我们鉴定了一种新的生殖细胞特异性转录因子，命名为Sohlh1(精子发生和卵子发生特异性碱性螺旋-环-螺旋(BHLH)转录因子)。在男性中，Sohlh1主要在前精原细胞和A型精原细胞中表达。Sohlh1的缺失会破坏精原细胞向精母细胞的分化，从而导致不育。7日龄无Sohlh1的睾丸仍表达睾丸特异转录因子ETV5、Taf4b、Zfp148和PLZF，过表达一种新的Sohlh2 bHLH转录因子，但缺乏LIM同源框基因Lhx8，表现为Ngn3表达降低。Sohlh1是第一个睾丸特异的bHLH转录因子，对精原细胞分化至关重要。(C)2006 Elsevier Inc.保留所有权利。
Spermatogonia are adult germline stem cells that can both self-renew and differentiate into spermatocytes. Little is known about factors necessary for spermatogonial differentiation. We identified a novel germ-cell-specific transcription factor that we named Sohlh1 (spermatogenesis and oogenesis specific basic helix-loop-helix (bHLH) transcription factor). In males, Sohlh1 is preferentially expressed in prespermatogonia and Type A spermatogonia. Loss of Sohlh1 causes infertility by disrupting spermatogonial differentiation into spermatocytes. Seven-day-old testes without Sohlh1 still express the testis-specific transcription factors Etv5, Taf4b, Zfp148, and Plzf, overexpress a novel Sohlh2 bHLH transcription factor, but lack LIM homeobox gene Lhx8 and show reduced expression of Ngn3. Sohlh1 represents the first testis-specific bHLH transcription factor that is essential for sperniatogonial differentiation. (c) 2006 Elsevier Inc. All rights reserved.