MesP1: a novel basic helix-loop-helix protein expressed in the nascent mesodermal cells during mouse gastrulation.
MesP1: a novel basic helix-loop-helix protein expressed in the nascent mesodermal cells during mouse gastrulation.
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发表时间：
1996-09
期刊：
影响因子：
4.6
通讯作者：
Y. Saga;Naomi Hata;Satoru Kobayashi;T. Magnuson;M. Seldin;M. Seldin;M. Taketo
中科院分区：
文献类型：
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作者：
Y. Saga;Naomi Hata;Satoru Kobayashi;T. Magnuson;M. Seldin;M. Seldin;M. Taketo
利用消减杂交技术分离小鼠原始生殖细胞（PGC）发育相关基因。在从尿囊基部分离的RNA上扩增互补DNA，其中PGC位于7.5天后交配（dpc）的小鼠胚胎中。然后通过与在PGC不存在的前部区域的RNA上扩增的cDNA杂交来减去它。一个新的基因，因此分离命名为Mesp1和编码一个可能的转录因子MesP1含有一个基本的螺旋环螺旋基序。其最早的表达观察到在原肠胚形成的发病，早在6.5 dpc，在新生的中胚层细胞，第一次进入在原始条纹的末端。这些表达细胞在侧胚层和胚外中胚层中呈翅状分布。在原肠胚形成前7.5 dpc，除胚外中胚层和尿囊基部的原条近端外，其初始表达迅速下调。在8dpc时，表达在尿囊基部的横向移动。这种分布在7.0和8.0 dpc之间与碱性磷酸酶活性检测到的PGC相似。而当PGC进入迁移期后，Mesp1的表达下调。出生后，Mesp1的表达只在成熟的睾丸中检测到，但在不同的亚型从胚胎中表达。Mesp1定位于第7号染色体的中部区域，靠近中胚层缺陷基因（mesd）。然而，Southern杂交研究清楚地表明，Mesp1与mesd明显不同。MesP1的氨基酸序列及其表达模式表明，MesP1在包括PGC在内的新生中胚层发育中起重要作用。
A subtractive hybridization strategy was used to isolate putative genes involved in the development of mouse primordial germ cells (PGC). Complimentary DNA was amplified on RNA isolated from the base of the allantois where PGC are located in the 7.5 days post coitum (dpc) mouse embryo. It was then subtracted by hybridization with cDNA amplified on RNA of the anterior region where PGC are absent. A novel gene thus isolated is designated as Mesp1 and encodes a possible transcription factor MesP1 containing a basic helix-loop-helix motif. Its earliest expression was observed at the onset of gastrulation, as early as 6.5 dpc, in the nascent mesodermal cells that first ingressed at the end of the primitive streak. These expressing cells in the lateral and extraembryonic mesoderm showed a wing-shaped distribution. Its initial expression was soon down-regulated at 7.5 dpc before the completion of gastrulation, except at the proximal end of the primitive streak which included the extraembryonic mesoderm and the base of allantois. At 8 dpc, the expression at the base of the allantois moved laterally. This distribution between 7.0 and 8.0 dpc was similar to that of PGC detected by the alkaline phosphatase activity. However, the expression of Mesp1 was down-regulated thereafter, when PGC entered in the migration stage. After birth, Mesp1 expression was detected only in mature testes, but in a different isoform from that expressed in the embryo. Mesp1 was mapped to the mid region of chromosome 7, near the mesodermal deficiency gene (mesd). However, a Southern hybridization study clearly showed that Mesp1 was distinctly different from mesd. The amino acid sequence and its expression pattern suggest that MesP1 plays an important role in the development of the nascent mesoderm including PGC.