Resolution of sensory and mucoid glycoconjugates with terminal alpha-galactose residues in the mucomicrovillar complex of the vomeronasal sensory epithelium by dual confocal laser scanning microscopy.
Resolution of sensory and mucoid glycoconjugates with terminal alpha-galactose residues in the mucomicrovillar complex of the vomeronasal sensory epithelium by dual confocal laser scanning microscopy.
复制标题
通过双共焦激光扫描显微镜解析犁鼻感觉上皮粘液微绒毛复合物中带有末端 α-半乳糖残基的感觉和粘液糖缀合物。
DOI：
10.1007/bf00307791
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发表时间：
1995
影响因子：
3.6
通讯作者：
Getchell,TV
中科院分区：
文献类型：
--
作者：
Takami,S;Getchell,ML;Getchell,TV
用共聚焦激光扫描显微镜观察了成年大鼠犁鼻感觉上皮粘微绒毛复合体的组织结构。用识别糖复合物末端α-半乳糖残基的Bandeiraea simplicifolia异凝集素B4标记的样品中，我们证明了粘微绒毛复合物由具有高密度α-半乳糖核心的胰岛样结构组成。在双标记和双扫描实验中，mucomicrovillar复合物进一步分解为感觉和粘液成分。感觉成分由嗅觉标记蛋白免疫反应性犁鼻器受体神经元的树突终末组成，其胞浆中含有末端为α-半乳糖残基的糖复合物。细胞外粘液成分由含有末端α-半乳糖的糖缀合物组成，该末端α-半乳糖来源于犁鼻器相关腺体。这些结果表明，mucomicrovillar复合体的感觉和粘液成分的复杂的微化学组织。
The organization of the mucomicrovillar complex of the vomeronasal sensory epithelium of adult rats was examined using confocal laser scanning microscopy. In specimens labeled with the FITC-conjugated isolectin B4ofBandeiraea simplicifolia, which recognizes terminal α-galactose sugar residues of glycoconjugates, we demonstrated that the mucomicrovillar complex was composed of islet-like structures with a high-density α-galactose core. The mucomicrovillar complex was further resolved into sensory and mucoid components in double-labeling and dual scanning experiments. The sensory component, which consists of the dendritic terminals of olfactory marker protein-immunoreactive vomeronasal receptor neurons, contained cytosolic glycoconjugates with terminal α-galactose sugar residues. The extracellular mucoid component consisted of glycoconjugates containing terminal α-galactose derived from the glands associated with the vomeronasal organ. These results demonstrated the complex microchemical organization of the sensory and mucoid components of the mucomicrovillar complex.