A dense network of dendritic cells populates the murine epididymis.
A dense network of dendritic cells populates the murine epididymis.
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DOI：
10.1530/rep-10-0493
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Breton S
中科院分区：
文献类型：
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作者：
Da Silva N;Cortez-Retamozo V;Reinecker HC;Wildgruber M;Hill E;Brown D;Swirski FK;Pittet MJ;Breton S
男性生殖生理学中最有趣的一个方面是产生生精细胞的能力——这对宿主来说是“外来的”——而不会触发免疫激活。离开睾丸后，精子进入附睾，在那里成熟并储存。我们在这里报告了一个以前未被识别的位于附睾上皮基部的密集树突状细胞网络。在表达CD11c-EYFP和CX3CR1-GFP报告基因的转基因小鼠中检测到该网络。附睾树突状细胞（eDCs）与上皮细胞建立密切的相互作用，并在上皮细胞之间向管腔投射长树突。我们发现，分离的eDCs表达许多白细胞标记物，这些白细胞标记物先前在与外部环境接触的其他器官中描述过，并且在体外向T细胞呈现和交叉呈现卵清蛋白。因此，eDCs具有调节免疫耐受和激活之间复杂相互作用的战略地位，这种平衡对男性生育能力至关重要。
One of the most intriguing aspects of male reproductive physiology is the ability to generate spermatogenic cells - which are “foreign” to the host - without triggering immune activation. After leaving the testis, spermatozoa enter the epididymis where they mature and are stored. We report here a previously unrecognized dense network of dendritic cells located at the base of the epididymal epithelium. This network was detected in transgenic mice expressing CD11c-EYFP and CX3CR1-GFP reporters. Epididymal dendritic cells (eDCs) establish intimate interactions with the epithelium and project long dendrites between epithelial cells toward the lumen. We show that isolated eDCs express numerous leukocyte markers described previously in other organs that are in contact with the external environment, and present and cross-present ovalbumin to T cells in vitro. eDCs are, therefore, strategically positioned to regulate the complex interplay between immune tolerance and activation, a balance that is fundamental to male fertility.