Near-Infrared Imaging of Serotonin Release from Cells with Fluorescent Nanosensors
Near-Infrared Imaging of Serotonin Release from Cells with Fluorescent Nanosensors
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DOI：
10.1021/acs.nanolett.9b02865
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发表时间：
2019-09-01
期刊：
影响因子：
10.8
通讯作者：
Kruss, Sebastian
中科院分区：
文献类型：
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作者：
Dinarvand, Meshkat;Neubert, Elsa;Kruss, Sebastian
血清素是一种重要的神经递质，参与神经、血液和免疫系统的各种功能。一般来说，传统的传感器和方法在高空间和时间分辨率下实时检测5 -羟色胺等小生物分子仍然具有挑战性。在这项工作中，我们设计了一种基于荧光单壁碳纳米管（SWCNTs）的近红外（nIR）荧光纳米传感器（NIRSer），用于实时成像人类血小板中血清素的释放。该纳米传感器由一个非漂白的swcnts骨架组成，该骨架在有益的近红外组织透明窗口（800-1700 nm）中荧光，以及一个5 -羟色胺结合DNA适体。NIRSer传感器的荧光（(6,5)-SWCNTs的发射波长为995 nm）对5 -羟色胺的响应增加了高达1.8倍。它以301 nM +/- 138 nM的解离常数和100 nM至1 μ m的生理相关区域的动态线性范围可逆地检测血清素。作为原理证明，我们在单细胞水平上检测了活化血小板的血清素释放模式。对血小板周围和血小板下方纳米传感器的成像使我们能够定位血清素释放的热点，并量化血小板中刺激和释放之间的时间延迟（大约21-30秒），突出了这种纳米传感器方法的时空分辨率。总之，我们报道了一种用于神经递质5 -羟色胺的近红外荧光纳米传感器，并展示了其在细胞间化学通讯成像方面的潜力。
Serotonin is an important neurotransmitter involved in various functions of the nervous, blood, and immune system. In general, detection of small biomolecules such as serotonin in real time with high spatial and temporal resolution remains challenging with conventional sensors and methods. In this work, we designed a near-infrared (nIR) fluorescent nanosensor (NIRSer) based on fluorescent single-walled carbon nanotubes (SWCNTs) to image the release of serotonin from human blood platelets in real time. The nanosensor consists of a nonbleaching SWCNT backbone, which is fluorescent in the beneficial nIR tissue transparency window (800-1700 nm) and a serotonin binding DNA aptamer. The fluorescence of the NIRSer sensor (995 nm emission wavelength for (6,5)-SWCNTs) increases in response to serotonin by a factor up to 1.8. It detects serotonin reversibly with a dissociation constant of 301 nM +/- 138 nM and a dynamic linear range in the physiologically relevant region from 100 nM to 1 mu M. As a proof of principle, we detected serotonin release patterns from activated platelets on the single-cell level. Imaging of the nanosensors around and under the platelets enabled us to locate hot spots of serotonin release and quantify the time delay (approximate to 21-30 s) between stimulation and release in a population of platelets, highlighting the spatiotemporal resolution of this nanosensor approach. In summary, we report a nIR fluorescent nanosensor for the neurotransmitter serotonin and show its potential for imaging of chemical communication between cells.