Specific developmental reductions in subventricular zone ErbB1 and ErbB4 mRNA in the human brain
Specific developmental reductions in subventricular zone ErbB1 and ErbB4 mRNA in the human brain
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DOI：
10.1016/j.ijdevneu.2008.06.004
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发表时间：
2008-11-01
影响因子：
1.8
通讯作者：
Weickert, Cynthia Shannon
中科院分区：
文献类型：
--
作者：
Chong, Victor Z.;Webster, Maree J.;Weickert, Cynthia Shannon
灵长类动物出生后脑室下区（SVZ）位于侧脑室腹外侧缘下，是一个由ErbB受体调控的胶质细胞和神经细胞组成的区域。然而，每种ErbB亚型在SVZ细胞发育中的具体作用仍不清楚，特别是在人脑中。ErbB亚型在人脑中的出生后空间和时间表达谱可能为出生后SVZ的独特功能提供有价值的见解。因此，我们研究了ErbB 1，ErbB 2，ErbB 3和ErbB 4 mRNA的表达谱的人死后的新生儿，婴儿，幼儿，学龄期受试者，青少年，年轻的成年人和成年人使用原位杂交的SVZ。SVZ的ErbB 1和ErbB 4转录水平在新生儿中最高，并随着年龄的增长而降低。SVZ ErbB 4 mRNA量在出生后第一年显著下降>85%至几乎检测不到的水平，而SVZ ErbB 1转录水平显示出更渐进的下降，在5岁后稳定至与新生儿水平的30-40%相似。在新生儿和婴儿SVZ中，ErbB 4 mRNA定位于类似于迁移性成神经细胞聚集体的细胞簇，而ErbB 1 mRNA在细胞中表达，沿着但不在这些簇内。ErbB 2 mRNA似乎在所有出生后年龄的人SVZ中持续表达，而ErbB 3转录本在出生后任何年龄的人SVZ中均未检测到。这些结果表明，ErbB 1和ErbB 4可能发挥更显着的作用比ErbB 2和ErbB 3在调解出生后早期的神经发育事件。此外，ErbB 1-和ErbB 4-免疫反应细胞和纤维广泛的整个人类婴儿SVZ，但似乎没有重叠的PSA-NCAM-免疫阳性集群。新生儿和婴儿年龄组SVZ ErbB 4表达的限制可能表明SVZ衍生的ErbB 4依赖性出生后神经元发育在出生后早期的狭窄时间范围内最广泛。(C)2008 ISDN。由爱思唯尔有限公司出版。保留所有权利。
The primate postnatal subventricular zone (SVZ) ties under the ventrolateral borders of the lateral ventricles as a discrete region of cells with gliogenic and neurogenic capacity regulated by ErbB receptors. However, the specific role of each ErbB subtype in SVZ cell development remains unclear, particularly in the human brain. The postnatal spatial and temporal expression profile of ErbB subtypes in the human brain may provide valuable insight into their distinct functions in the SVZ following birth. Hence, we examined the expression profile of ErbB1, ErbB2, ErbB3 and ErbB4 mRNA in the SVZ of human postmortem brains from neonates, infants, toddlers, school age subjects, adolescents, young adults and adults using in situ hybridization. SVZ transcript levels of ErbB1 and ErbB4 were highest in neonates and diminished with age. SVZ ErbB4 mRNA quantities significantly decreased by >85% to almost undetectable levels after the first year of life, while SVZ ErbB1 transcript levels displayed more gradual reductions, stabilizing to similar to 30-40% of neonate levels after the age of 5 years. In the neonate and infant SVZ, ErbB4 mRNA was localized to cell clusters resembling migratory neuroblast aggregates whereas ErbB1 mRNA was expressed in cells along but not within these clusters. ErbB2 mRNA appeared to be constantly expressed in the human SVZ at all postnatal ages as opposed to ErbB3 transcripts, which were not detected in the human SVZ at any age following birth. These findings suggest that ErbB1 and ErbB4 may play more salient roles than ErbB2 and ErbB3 in mediating early postnatal neurodevelopmental events. In addition, ErbB1- and ErbB4-immunoreactive cells and fibers were extensive throughout the human infant SVZ, but did not appear to overlap with PSA-NCAM-immunopositive clusters. The restriction of robust SVZ ErbB4 expression to neonate and infant age groups may indicate that SVZ-derived ErbB4-dependent postnatal neuronal development is most extensive within a narrow time frame early after birth. (C) 2008 ISDN. Published by Elsevier Ltd. All rights reserved.