Hypertension, calcium channel and pyridoxine (vitamin B6)
Hypertension, calcium channel and pyridoxine (vitamin B6)
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DOI：
10.1023/a:1006832810292
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发表时间：
1998-11-01
影响因子：
4.3
通讯作者：
Ganguly, PK
中科院分区：
文献类型：
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作者：
Dakshinamurti, K;Lal, KJ;Ganguly, PK
我们引进中度缺乏维生素B-6的雄性大鼠作为高血压研究的动物模型(B6DHT)。这只大鼠的高血压与交感神经刺激的增加有关。B6DHT大鼠的动脉节段维持较高的静息张力。B6DHT大鼠尾状动脉血管平滑肌细胞内~(45)Ca内流也明显增加。服用吡哆醇可降低B6DHT大鼠的高血压，以及遗传或饮食诱导的中度高血压条件下的高血压，如Zucker肥胖大鼠和蔗糖或低钙喂养的大鼠。然而，吡哆醇对自发性高血压大鼠没有任何影响。各种钙通道阻滞剂均能有效降低B6DHT大鼠的收缩压。磷酸吡哆醛和二氢吡啶敏感的钙通道阻断剂(DHP)可阻断B6DHT大鼠动脉节段体外钙离子内流增加和Bay K 8644诱导的正常大鼠动脉节段钙离子内流。磷酸吡哆醛(PLP)在体外可增强钙通道拮抗剂与血管组织膜制剂的结合。PLP可纠正反应性高血压时的膜异常，因此可能是DHP敏感性钙通道的内源性调节剂。
The moderately pyridoxine (vitamin B-6)-deficient male rat was introduced by us as an animal model (B6DHT) for the study of hypertension. Hypertension in this rat is associated with increased sympathetic stimulation. Arterial segments from B6DHT rats maintained a higher resting tone. The influx of (45)calcium into intracellular compartment of the vascular smooth muscle of the caudate artery of B6DHT rats was also enhanced. Administration of pyridoxine attenuated the hypertension in B6DHT rats as well as in genetic or dietary-induced moderately hypertensive conditions such as in the Zucker obese rat and sucrose or low calcium-fed rats. However, pyridoxine did not have any effect on the spontaneously hypertensive rat. All classes of calcium channel blockers were effective in lowering the systolic blood pressure of B6DHT rats. The increased in vitro influx of (45)calcium into intracellular compartment of artery segments of B6DHT rats as well as the BAY K 8644-induced influx of (45)calcium into artery segments from normal rats were blocked by pyridoxal phosphate as well as by dihydropyridine-sensitive calcium channel blockers (DHP). Pyridoxal phosphate (PLP) in vitro enhances the binding of calcium channel antagonists to membrane preparations from vascular tissue. PLP corrects the membrane abnormality in responsive hypertensive conditions and thus, could be an endogenous modulator of DHP - sensitive calcium channels.