NOVEL JUN-RELATED AND FOS-RELATED PROTEINS IN DROSOPHILA ARE FUNCTIONALLY HOMOLOGOUS TO ENHANCER FACTOR AP-1
NOVEL JUN-RELATED AND FOS-RELATED PROTEINS IN DROSOPHILA ARE FUNCTIONALLY HOMOLOGOUS TO ENHANCER FACTOR AP-1
复制标题
DOI：
10.1002/j.1460-2075.1988.tb03324.x
复制
发表时间：
1988-12-20
期刊：
影响因子：
11.4
通讯作者：
TJIAN, R
中科院分区：
文献类型：
--
作者：
PERKINS, KK;DAILEY, GM;TJIAN, R
在果蝇中检测到哺乳动物增强子结合因子AP-1的同源物，并通过序列特异性DNA亲和层析从胚胎核提取液中纯化了AP-1。纯化的组分DAP-1具有哺乳动物AP-1的序列特异性和转录激活特性，由两种主要的摩尔蛋白质组成。WTS 40和70kd。抗体交叉反应实验表明，这些蛋白是果蝇原癌基因产物Jun和Fos的同源物。果蝇Jun和Fos相关抗原被分离后，分别能够与序列特异的DNA结合，Jun相关抗原在体外激活转录。
A homolog of mammalian enhancer binding factor AP-1 was detected in Drosophila and was purified from embryo nuclear extracts by sequence-specific DNA affinity chromatography. The purified fraction, dAP-1, displays the sequence specificity as well as transcriptional activation properties of mammalian AP-1 and consists of two major proteins of mol. wts 40 and 70 kd. Antibody cross-reactivity experiments suggest that these proteins are Drosophila homologs of proto-oncogene products, Jun and Fos. The Drosophila Jun- and Fos-related antigens, when separated, are individually capable of sequence-specific DNA binding, and the Jun-related antigen activates transcription in vitro.