IL28B genetic variants and gender are associated with spontaneous clearance of hepatitis C virus infection
IL28B genetic variants and gender are associated with spontaneous clearance of hepatitis C virus infection
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IL28B 基因变异和性别与丙型肝炎病毒感染的自发清除相关
DOI：
10.1111/j.1365-2893.2011.01497.x
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发表时间：
2012-03-01
影响因子：
2.5
通讯作者：
Wei, L.
中科院分区：
文献类型：
--
作者：
Rao, H. -Y.;Sun, D. -G.;Wei, L.
。IL28B基因附近的单核苷酸多态(SNPs)已被证明与慢性丙型肝炎的治疗反应和丙型肝炎病毒感染的自发清除有关。我们分析了376名感染丙型肝炎病毒的中国有偿血浆献血员中IL28B基因变异与丙型肝炎病毒感染自发清除的关系。用IPLEX系统(MassARRAY(R)SNP基因分型；Sequenom)对所有献血者IL28B区附近的8个SNP进行基因分型，并对所有80名清除了丙型肝炎病毒的献血者和296名未清除丙型肝炎病毒的献血者中的160人进行测序，以验证基因分型。80例(21.3%)献血者自发清除了丙型肝炎病毒。Rs8099917TT(VsGT)、rs8105790TT(VsCT)、rs12980275AA(VsAG)和rs10853728CC(vsCG或GG)4个SNP与自发丙型肝炎病毒清除显著相关，其OR(95%CI)分别为15.27(2.07112.50)、14.88(2.02109.72)、7.92(1.8833.32)和2.32(1.224.42)。未发现包括rs12979860在内的其他4个IL28B SNPs与自发的丙型肝炎病毒清除有关。女性丙型肝炎自发转阴率高于男性[56/213(26.3%)vs24/163(14.6%)，P=0.007]，即使在对IL28B基因进行分层后，优势基因携带者的OR值为1.92.2。我们的结果证实了IL28B基因多态性与中国人群中丙型肝炎病毒的自发清除有关，但预测中国人群中丙型肝炎病毒清除的SNPs与高加索人群中报道的不同。无论IL28B基因分型如何，女性更有可能清除丙型肝炎病毒感染。
. Single nucleotide polymorphisms (SNPs) near the IL28B gene have been shown to be associated with response to treatment for chronic hepatitis C and also with spontaneous clearance of hepatitis C virus (HCV) infection. We analysed the association between IL28B genetic variants and spontaneous clearance of HCV infection in 376 HCV-infected Chinese paid plasma donors. Genotyping of eight SNPs near the IL28B region was performed by the iPLEX system (MassARRAY (R) SNP Genotyping; Sequenom) in all donors, and sequencing was performed on all 80 donors who cleared HCV and on 160 of 296 donors who did not clear HCV to validate the genotypes. Eighty (21.3%) donors spontaneously cleared HCV. Four SNPs were significantly associated with spontaneous HCV clearance: rs8099917 TT (vs GT), rs8105790 TT (vs CT), rs12980275 AA (vs AG) and rs10853728 CC (vs CG or GG) with OR (95% CI) 15.27 (2.07112.50), 14.88 (2.02109.72), 7.92 (1.8833.32) and 2.32 (1.224.42) respectively. No association between the other four IL28B SNPs including rs12979860 and spontaneous HCV clearance was found. Women had a higher rate of spontaneous HCV clearance than men [56/213 (26.3%) vs 24/163 (14.6%), P = 0.007], and this was true even after stratification for IL28B genotypes with OR of 1.92.2 among those with favourable genotypes. Our results confirmed that IL28B polymorphism is associated with spontaneous clearance of HCV in Chinese subjects, but the SNPs that predict HCV clearance in Chinese subjects were different from those reported in Caucasians. Women were more likely to clear HCV infection regardless of IL28B genotypes.