Comparative analysis of the UDP-glycosyltransferase multigene family in insects
Comparative analysis of the UDP-glycosyltransferase multigene family in insects
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DOI：
10.1016/j.ibmb.2011.11.006
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发表时间：
2012-02-01
影响因子：
3.8
通讯作者：
Heckel, David G.
中科院分区：
文献类型：
--
作者：
Ahn, Seung-Joon;Vogel, Heiko;Heckel, David G.
UDP-糖基转移酶（UGT）催化一系列小分子亲脂性化合物与糖结合生成糖苷，在昆虫体内外源物质的解毒和内源生物素的调节中发挥重要作用。基因组测序的最新进展使得能够评估昆虫中UGT多基因家族的范围。在这里，我们报告了超过310个推定的UGT基因，从8个不同的昆虫物种的基因组数据库，以及从鳞翅目棉铃虫的转录本数据库。昆虫UGT的系统发育分析表明，该多基因家族的目的特异性基因多样性和种间保守性。只有一个家庭（UGT 50）被发现在所有调查的昆虫物种（豌豆蚜虫除外），可能是同源的哺乳动物UGT 8。与鳞翅目UGT相关的三个家族（UGT 31、UGT 32和UGT 305）是杆状病毒所特有的。用40个H.对棉铃虫UGT和44个家蚕UGT的研究表明，两个物种中某些家族的谱系特异性扩增似乎是由N-末端底物结合结构域的多样化驱动的，增加了可以解毒或通过糖基化调节的化合物的范围。通过比较推导的蛋白序列，预测了几个重要的结构域，包括N端信号肽、UGT特征基序和C端跨膜结构域。此外，通过与人UGT的比较，还鉴定了几个参与糖供体结合和催化机制的保守残基。许多UGT在脂肪体、中肠和马氏管中表达，与解毒功能一致，一些UGT在触角中表达，表明在信息素失活中的作用。来自选择性剪接、外显子跳跃或内含子保留的转录变体产生额外的UGT多样性。来自两种鳞翅目UGT以及其他昆虫的比较研究的这些发现揭示了与脊椎动物、植物和真菌中的该基因家族相当的多样性，并显示了未来任务的重要性，以确定每种UGT酶的生化功能和生理相关性。(C)2011爱思唯尔有限公司保留所有权利。
UDP-glycosyltransferases (UGT) catalyze the conjugation of a range of diverse small lipophilic compounds with sugars to produce glycosides, playing an important role in the detoxification of xenobiotics and in the regulation of endobiotics in insects. Recent progress in genome sequencing has enabled an assessment of the extent of the UGT multigene family in insects. Here we report over 310 putative UGT genes identified from genomic databases of eight different insect species together with a transcript database from the lepidopteran Helicoverpa armigera. Phylogenetic analysis of the insect UGTs showed Order-specific gene diversification and inter-species conservation of this multigene family. Only one family (UGT50) is found in all insect species surveyed (except the pea aphid) and may be homologous to mammalian UGT8. Three families (UGT31, UGT32, and UGT305) related to Lepidopteran UGTs are unique to baculoviruses. A lepidopteran sub-tree constructed with 40 H. armigera UGTs and 44 Bombyx mori UGTs revealed that lineage-specific expansions of some families in both species appear to be driven by diversification in the N-terminal substrate binding domain, increasing the range of compounds that could be detoxified or regulated by glycosylation. By comparison of the deduced protein sequences, several important domains were predicted, including the N-terminal signal peptide, UGT signature motif, and C-terminal transmembrane domain. Furthermore, several conserved residues putatively involved in sugar donor binding and catalytic mechanism were also identified by comparison with human UGTs. Many UGTs were expressed in fat body, midgut, and Malpighian tubules, consistent with functions in detoxification, and some were expressed in antennae, suggesting a role in pheromone deactivation. Transcript variants derived from alternative splicing, exon skipping, or intron retention produced additional UGT diversity. These findings from this comparative study of two lepidopteran UGTs as well as other insects reveal a diversity comparable to this gene family in vertebrates, plants and fungi and show the magnitude of the task ahead, to determine biochemical function and physiological relevance of each UGT enzyme. (C) 2011 Elsevier Ltd. All rights reserved.