Rapid purification of host-specific pathotoxins from Alternaria alternata f. sp. lycopersici by solid-phase adsorption on octadecylsilane.
Rapid purification of host-specific pathotoxins from Alternaria alternata f. sp. lycopersici by solid-phase adsorption on octadecylsilane.
复制标题
快速纯化链格孢菌 (Alternaria alternata f) 中的宿主特异性病原体。
DOI：
10.1016/s0021-9673(01)93524-1
复制
发表时间：
1985
影响因子：
4.1
通讯作者：
D. Gilchrist
中科院分区：
文献类型：
--
作者：
S. Clouse;A. Martensen;D. Gilchrist
一种从赤星病菌无细胞培养滤液中分离纯化两种植物毒性代谢物(TA和TB)的简便、快速的方法。番茄红假单胞菌在大孔、C18键合硅胶上进行疏水层析，然后进行凝胶过滤。在最终制备中实现了TA和TB的基线分离，产率达到80%，并在所有测试系统中保留了全部生物活性。用核磁共振和高效液相色谱对毒素进行分析，表明TA的纯度至少为99%。该程序的效率允许快速积累纯TA和TB，用于研究这种新的寄主特有的植物毒素的分子作用模式和寄主植物的反应。
A simplified, rapid procedure for the purification of two phytotoxic metabolites (TA and TB) from cell-free culture filtrates ofAlternaria alternataf. sp.lycopersiciwas developed using hydrophobic chromatography on macroporous, C18bonded silica followed by gel filtration. Baseline separation of TA from TB was achieved in the final preparation and yields of 80% were obtained with retention of full biological activity in all assay systems tested. Toxin analysis by nuclear magnetic resonance and high-performance liquid chromatography indicated TA was at least 99% pure. The efficiency of the procedure allows rapid accumulation of pure TA and TB for studies on the molecular mode of action and host plant response to this novel host-specific phytotoxin.