Chronology of the appearance of beta, A, and alpha mitochondria-rich cells in the gill epithelium during ontogenesis of the brown trout (Salmo trutta).
Chronology of the appearance of beta, A, and alpha mitochondria-rich cells in the gill epithelium during ontogenesis of the brown trout (Salmo trutta).
复制标题
褐鳟（Salmo trutta）个体发育过程中鳃上皮中富含 β、A 和 α 线粒体的细胞出现的时间顺序。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
P. Prunet
中科院分区：
文献类型：
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作者：
M. Pisam;F. Massa;C. Jammet;P. Prunet
在淡水硬骨鱼幼鱼和成鱼鳃上皮中观察到三种类型的富甲细胞，即α细胞、β细胞和辅助细胞。除了大量的线粒体，它们的细胞质中还含有一个连接到侧基底质膜的膜小管网络，即管状系统。因为它们被认为在离子调节中发挥作用，所以有兴趣检查这些细胞在褐鳟鱼胚胎发生和幼虫期的外观顺序和超微结构特征。因此，在不同的阶段，去除胚胎和鱼苗的鳃保持在淡水中，并准备透射和扫描电子显微镜检查。孵化前一周，细胞类似的β细胞的少年和成年硬骨鱼首先出现在上皮细胞位于在鳃的细丝的基础上的褐鳟鱼幼虫。除了它们的管状系统外，它们还含有许多大型的顶端结构，似乎起源于高尔基体。在大约孵化时，观察到小梨形细胞与β细胞的侧面紧密贴壁;它们通常没有顶端结构，被认为是辅助细胞。卵黄囊吸收后，沿板层沿着出现额外的细胞，即α细胞。与β细胞相反，它们仅表现出发育不良的顶端结构。这三种类型的MR细胞在鱼的发育过程中的调节的可能作用进行了讨论。
Three types of mitochondria-rich (MR) cells, the alpha, beta, and accessory cells, are observed in the gill epithelium of juvenile and adult freshwater teleosts. In addition to numerous mitochondria, their cytoplasm contains a network of membranous tubules, the tubular system, connected to the laterobasal plasma membrane. Because they are believed to play a role in ionic regulation, it is of interest to examine the order of appearance and the ultrastructural characteristics of such cells during the embryogenesis and larval life of the brown trout. Gills of embryos and fry maintained in freshwater were thus removed at different stages and prepared for transmission and scanning electron microscopic examination. One week before hatching, cells resembling the beta cells of juvenile and adult teleosts appeared first among the epithelial cells located at the base of the filaments in the gills of the brown trout larva. In addition to their tubular system, they contained numerous and large apical structures seemingly originating from the Golgi apparatus. At approximately hatching time, small pear-shaped cells were seen to be closely apposed to the lateral side of the beta cells; they were usually devoid of apical structures and were considered to be accessory cells. After yolk sac resorption, additional cells, the alpha cells, were present along the lamellae. In contrast to the beta cells, they only exhibited poorly developed apical structures. The possible role of these three types of MR cells in osmoregulation during fish development is discussed.