Time Evolution of the Nanoparticle Protein Corona
Time Evolution of the Nanoparticle Protein Corona
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DOI：
10.1021/nn901372t
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发表时间：
2010-07-01
期刊：
影响因子：
17.1
通讯作者：
Puntes, Victor
中科院分区：
文献类型：
--
作者：
Casals, Eudald;Pfaller, Tobias;Puntes, Victor
在这项工作中，我们探索了金属金纳米颗粒（NPs）暴露于含有10%胎牛血清的细胞培养基后蛋白质冠的形成，其大小范围从4到40 nm。在体外细胞培养条件下，通过zeta电位测量、紫外-可见光谱、动态光散射和透射电镜分析，监测无机NP蛋白电晕形成的时间演变，表征实验各阶段NPs的稳定性和表面状态。表面等离子体共振峰的红移以及表面电荷的下降和流体动力直径的增加表明蛋白质与NPs结合。值得注意的是，随着时间的推移，观察到从松散附着到不可逆附着的蛋白质冠的进化。酶解蛋白冠质谱分析显示白蛋白是最丰富的成分，表明其生物相容性得到改善。
In this work, we explore the formation of the protein corona after exposure of metallic Au nanoparticles (NPs), with sizes ranging from 4 to 40 nm, to cell culture media containing 10% of fetal bovine serum. Under in vitro cell culture conditions, zeta potential measurements, UV-vis spectroscopy, dynamic light scattering and transmission electron microscope analysis were used to monitor the time evolution of the inorganic NP protein corona formation and to characterize the stability of the NPs and their surface state at every stage of the experiment. As expected, the red-shift of the surface plasmon resonance peak, as well as the drop of surface charge and the increase of the hydrodynamic diameter indicated the conjugation of proteins to NPs. Remarkably, an evolution from a loosely attached toward an irreversible attached protein corona over time was observed. Mass spectrometry of the digested protein corona revealed albumin as the most abundant component which suggests an improved biocompatibility.