Sol-Gel Derived Nanoporous Compositions for Entrapping Small Molecules and Their Outlook toward Aptamer Screening
Sol-Gel Derived Nanoporous Compositions for Entrapping Small Molecules and Their Outlook toward Aptamer Screening
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DOI：
10.1021/ac202559w
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发表时间：
2012-03-20
影响因子：
7.4
通讯作者：
Kim, Soyoun
中科院分区：
文献类型：
--
作者：
Ahn, Ji-Young;Lee, SangWook;Kim, Soyoun
本文首次报道了溶胶-凝胶微阵列在固定不溶性小分子化合物（双酚A; BPA）中的应用。此外，通过将溶胶-凝胶微点锚定到多孔硅表面（所谓的PS-SG芯片）上，避免了溶胶-凝胶粘附到常规微量滴定孔板基底上的已知问题。我们证实了低分子量的化学固定内的溶胶-凝胶网络使用荧光素。BPA和BPA特异性适体被用作模型对以验证PS-SG选择系统中的亲和特异性相互作用。适体在溶胶-凝胶斑点中与BPA特异性相互作用，如形成字母“L”、“U”、“N”和“D”的微阵列所示。此外，结合的适体通过加热释放，回收，并通过凝胶电泳验证。开发的PS-SG芯片平台将用于筛选针对许多小分子（如毒素、代谢物或农药残留）的适体。
This paper reports for the first time the application of sol-gel microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol-gel adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol-gel microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol-gel network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol-gel spots, as shown in microarrays forming the letters "L", "U", "N", and "D". Moreover, the bound aptamer was released by heat, recovered, and verified by gel electrophoresis. The developed PS-SG chip platform will be used for screening aptamers against numerous small molecules such as toxins, metabolites, or pesticide residues.