A study of the effect of general anesthetics on lipid-protein interactions in acetylcholine receptor enriched membranes from Torpedo nobiliana using nitroxide spin-labels.
A study of the effect of general anesthetics on lipid-protein interactions in acetylcholine receptor enriched membranes from Torpedo nobiliana using nitroxide spin-labels.
复制标题
使用硝基氧自旋标记研究全身麻醉剂对来自鱼雷鱼雷受体的富含乙酰胆碱受体的膜中脂质-蛋白质相互作用的影响。
DOI：
10.1021/bi00463a007
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Watts,A
中科院分区：
文献类型：
--
作者：
Fraser,DM;Louro,SR;Horváath,LI;Miller,KW;Watts,A
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Materials and MethodsPreparation of Membranes. Nicotinic acetylcholine receptor (nAcChoR) rich membranes were obtained from the electric organ of Torpedo nobiliana by differential and sucrose density gradient centrifugation according to procedures adapted from Cohen et al.(1972). Thebuffer used was Ca2+-free Torpedo Ringer (TR), pH 7.4. Membranes, at a total protein con-centration of 10 mg-mL'1 and specific activity of 1.2 µ of nAcChoR-(g of protein)" 1, were stored at-70 C in 0.3-mL aliquots.