The CCoAOMT1 gene from jute (Corchorus capsularis L.) is involved in lignin biosynthesis in Arabidopsis thaliana.
The CCoAOMT1 gene from jute (Corchorus capsularis L.) is involved in lignin biosynthesis in Arabidopsis thaliana.
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DOI：
10.1016/j.gene.2014.05.011
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发表时间：
2014-08
期刊：
影响因子：
3.5
通讯作者：
Gaoyang Zhang;Yujia Zhang;Jiantang Xu;Xiaoping Niu;J. Qi;A. Tao;Liwu Zhang;Pingping Fang;
中科院分区：
文献类型：
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作者：
Gaoyang Zhang;Yujia Zhang;Jiantang Xu;Xiaoping Niu;J. Qi;A. Tao;Liwu Zhang;Pingping Fang;
咖啡酰辅酶A3-O-甲基转移酶（CCoAOMT）是植物木质素生物合成的关键酶。本研究利用同源克隆法（根据其它植物咖啡酰辅酶A3-O-甲基转移酶（CCoAOMT）基因序列设计引物）和改良的RACE技术，从黄麻中克隆了CCoAOMT基因的全长cDNA，命名为“CcCCoAOMT 1”。生物信息学分析表明该基因属于CCoAOMT基因家族。Real-time PCR分析表明，CcCCoAOMT 1基因在所有组织中均组成型表达，其中茎中表达量最高，茎皮、根和叶次之。为了了解该基因的功能，我们将其转化到拟南芥中; PCR和Southern杂交后证实了整合（一个插入位点）。在这些转基因拟南芥植株中过表达CcCCoAOMT 1导致相对于非转基因植株的植株高度和角果长度增加。最重要的发现是转基因拟南芥植株中木质素含量（20.44-21.26%）高于对照植株（17.56%），表明CcCCoAOMT 1基因在木质素合成中起重要作用。这些数据对于通过CcCCoAOMT 1基因抑制来降低黄麻木质素含量（从而提高纤维品质）的努力的成功是重要的。
The Caffeoyl-CoA 3-O-methyltransferase (CCoAOMT) is a key enzyme in lignin biosynthesis in plants. In this study we cloned the full-length cDNA of the Caffeoyl-CoA 3-O-methyltransferase (CCoAOMT) gene from jute using homology clone (primers were designed according to the sequence ofCCoAOMTgene of other plants), and a modified RACE technique, subsequently named “CcCCoAOMT1”. Bioinformatic analyses showed that the gene is a member of theCCoAOMTgene family. Real-time PCR analysis revealed that theCcCCoAOMT1gene is constitutively expressed in all tissues, and the expression level was greatest in stem, followed by stem bark, roots and leaves. In order to understand this gene's function, we transformed it into Arabidopsis thaliana; integration (one insertion site) was confirmed following PCR and southern hybridization. The over-expression ofCcCCoAOMT1in these transgenicA.thalianaplants resulted in increased plant height and silique length relative to non-transgenic plants. Perhaps the most important finding was that the transgenicArabidopsisplants contained more lignin (20.44–21.26%) than did control plants (17.56%), clearly suggesting an important role ofCcCCoAOMT1gene in lignin biosynthesis. These data are important for the success of efforts to reduce jute lignin content (thereby increasing fiber quality) viaCcCCoAOMT1gene inhibition.