Brassinosteroid regulates secondary metabolism in tomato towards enhanced tolerance to phenanthrene
Brassinosteroid regulates secondary metabolism in tomato towards enhanced tolerance to phenanthrene
复制标题
油菜素类固醇调节番茄的次生代谢以增强对菲的耐受性
DOI：
10.1007/s10535-012-0128-9
复制
发表时间：
2013-03-01
影响因子：
1.5
通讯作者：
Yu, J. Q.
中科院分区：
文献类型：
--
作者：
Ahammed, G. J.;Zhou, Y. H.;Yu, J. Q.
研究了24-表油菜素内酯(EBR)在缓解番茄(Solanum lycopsicum L.)菲胁迫中的作用。300亩M的苯丙氨酸处理显著降低了番茄植株的地上部和根长(19%和16%)、鲜重(35%和43%)、叶绿素a(26%)、叶绿素b(27%)和类胡萝卜素(18%)的含量。此外，Phe还提高了次生代谢相关酶谷胱甘肽-S转移酶、葡萄糖-6-磷酸脱氢酶、莽草酸脱氢酶、苯丙氨酸解氨酶和肉桂醇脱氢酶的活性和丙二醛含量(57%)。Phe还诱导了GST1、PPO、SKDH、PAL和CAD基因的表达。重要的是，EBR(0.1亩M)单独和与Phe配施分别比对照和Phe单独显著提高了这些酶的生长、生物量和活性。与酶活性一致，Phe+EBR的GST1、PPO、SKDH、PAL和CAD的转录水平比单独Phe进一步增加。然而，Phe+EBR组的丙二醛含量显著低于单独使用Phe组。同时，苯丙氨酸还能提高总酚、总黄酮类化合物的含量和抗氧化活性，且苯丙氨酸+EBR进一步提高了这些指标。这些观察结果表明，EBR调节番茄的次生代谢，这可能会增强番茄对Phe的耐受性。
We investigated the role of 24-epibrassinolide (EBR) in the amelioration of phenanthrene (PHE) stress in tomato (Solanum lycopersicum L.). Exposure to PHE (300 mu M) significantly decreased shoot and root length (19 and 16 %, respectively), fresh mass (35 and 43 %, respectively), contents of chlorophyll a (26 %), chlorophyll b (27 %) and carotenoids (18 %) in tomato plants. In addition, PHE increased the malondialdehyde (MDA) content (57 %) and activity of secondary metabolism related enzymes glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PDH), shikimate dehydrogenase (SKDH), phenylalanine ammonia-lyase (PAL) and cinnamyl alcohol dehydrogenase (CAD). The expression levels of GST1, PPO, SKDH, PAL and CAD genes were also induced by PHE. Importantly, EBR (0.1 mu M) alone and in combination with PHE increased the growth, biomass and activity of those enzymes significantly over control and PHE alone, respectively. Consistent with enzymes activities transcript levels of GST1, PPO, SKDH, PAL and CAD were further increased in PHE+EBR over PHE alone. However, MDA content was remarkably decreased in PHE+EBR than PHE alone. Meanwhile, content of phenols, flavonoids and antioxidant activity were increased by PHE and PHE+EBR further increased all those parameters. These observations suggest that EBR regulates secondary metabolism in tomato which might enhance tolerance to PHE.