Behavioral effects of bovine lactoferrin administration during postnatal development of rats
Behavioral effects of bovine lactoferrin administration during postnatal development of rats
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DOI：
10.1007/s10534-014-9735-6
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发表时间：
2014-04
期刊：
影响因子：
3.5
通讯作者：
J. Shumake;Douglas W. Barrett;M. Lane;A. Wittke
中科院分区：
文献类型：
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作者：
J. Shumake;Douglas W. Barrett;M. Lane;A. Wittke
我们测试的假设，大鼠消耗牛乳铁蛋白（bLf）在出生后的发展将显示出更好的性能在青春期的压力任务。在第一项研究中，我们在出生后第16-34天每天一次口服给予bLf（750 mg/kg）。然后对大鼠进行一系列行为测试：旷场（被迫探索危险环境）、明暗浮现（自愿探索危险环境）、诱饵洞板（工作记忆和参考记忆）、食物新恐惧症（偏好熟悉的食物与新食物）、强迫游泳（抗抑郁功效测试）和梭箱逃脱（学习逃避脚震）。补充bLF的大鼠表现出对危险环境的探索较少，对熟悉食物气味的偏好更大，逃避反应更快。强迫游泳行为的bLf的效果取决于性别：不动性增加男性和女性减少。在下一项研究中，我们用逃脱游泳测试取代了强迫游泳测试，在该测试中，大鼠学会了使用视觉提示来定位逃脱平台，并且我们测试了bLf在该测试和梭箱逃脱测试中的剂量反应，受试者接受500、1，000或2，000 mg/kg的溶剂或bLf。在该改良的试验组合中，在任何剂量下均未观察到足电击逃逸的改善。然而，男性，而不是女性，表现出显着的剂量依赖性影响的bLf收购的水逃逸任务。平均而言，接受较高剂量的雄性比接受较低剂量或溶剂的大鼠快20- 25%掌握任务。这些结果提供了初步证据，表明在发育过程中补充bLf可以改善随后在压力下的认知表现。
We tested the hypothesis that rats consuming bovine lactoferrin (bLf) during postnatal development would show better performance of stressful tasks during adolescence. In the first study, we orally administered bLf (750 mg/kg) once daily between postnatal days 16–34. Rats then underwent a battery of behavioral tests: open field (forced exploration of risky environment), light–dark emergence (voluntary exploration of risky environment), baited holeboard (working and reference memory), food neophobia (preference for familiar versus novel food), forced swim (test for antidepressant efficacy), and shuttle-box escape (learning to escape footshock). bLf-supplemented rats showed less exploration of the risky environment, greater preference for the familiar food odor, and faster escape responses. The effect of bLf on forced-swim behavior depended on sex: immobility increased for males and decreased for females. In the next study, we replaced the forced-swim test with an escape-swim test in which rats learned to use a visual cue to locate an escape platform, and we tested the dose response of bLf on this and the shuttle-box escape test, with subjects receiving vehicle or bLf at 500, 1,000, or 2,000 mg/kg. Under this modified testing battery, improvement of escape from footshock was not observed at any dose. However, males, but not females, showed a significant dose-dependent effect of bLf on acquisition of the water-escape task. On average, males receiving a higher dose mastered the task 20–25 % sooner than rats receiving a lower dose or vehicle. These results offer preliminary evidence that bLf supplementation during development can improve subsequent cognitive performance during stress.