Interplay between Lefty and Nodal signaling is essential for the organizer and axial formation in amphioxus embryos
Interplay between Lefty and Nodal signaling is essential for the organizer and axial formation in amphioxus embryos
复制标题
Lefty和Nodal信号之间的相互作用对于文昌鱼胚胎的组织和轴向形成至关重要
DOI：
10.1016/j.ydbio.2019.08.006
复制
发表时间：
2019
影响因子：
2.7
通讯作者：
Guang Li
中科院分区：
文献类型：
--
作者：
Huayang Zhang;Shuang Chen;Chaoqi Shang;Xiaotong Wu;Yiquan Wang;Guang Li
组织者是脊椎动物胚胎发育中轴形成所必需的信号中心。在文昌鱼的基本脊索动物中，原肠胚的背部胚孔嘴唇被认为与脊椎动物的组织者同源。Lefty是组织者中最早表达的基因之一。结果表明，左侧过表达取消了组织者，胚胎严重腹侧化和后部化，不能发育出前部和背部结构。在左旋击倒中，组织者扩大，前部和背部结构扩张。与脊椎动物的左型变异体不同，文昌鱼左型突变体也表现出左向右向的缺陷。SB505124对Nodal的抑制可部分挽救左功能丧失对细胞形态的影响。另外，SB505124处理抑制了文昌鱼胚胎卵裂期的Lefty型表达，而激活蛋白激活Nodal信号诱导了这些时期的Lefty型异位表达。这些结果表明，Lefty和Nodal信号之间的相互作用在文昌鱼组织者和轴的指定中起着至关重要的作用。
The organizer is an essential signaling center required for axial formation during vertebrate embryonic development. In the basal chordate amphioxus, the dorsal blastopore lip of the gastrula has been proposed to be homologous to the vertebrate organizer.Leftyis one of the first genes to be expressed in the organizer. The present results show thatLeftyoverexpression abolishes the organizer; the embryos were severely ventralized and posteriorized, and failed to develop anterior and dorsal structures. InLeftyknockouts the organizer is enlarged, and anterior and dorsal structures are expanded. Different fromLeftymorphants in vertebrates, amphioxusLeftymutants also exhibited left-right defects. Inhibition of Nodal with SB505124 partially rescued the effects ofLeftyloss-of-function on morphology. In addition, while SB505124 treatment blockedLeftyexpression in the cleavage stages of amphioxus embryos, activation of Nodal signaling with Activin protein induced ectopicLeftyexpression at these stages. These results show that the interplay between Lefty and Nodal signaling plays an essential role in the specification of the amphioxus organizer and axes.