A biocompatible micro cell culture chamber (μCCC) for the culturing and on-line monitoring of eukaryote cells
A biocompatible micro cell culture chamber (μCCC) for the culturing and on-line monitoring of eukaryote cells
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DOI：
10.1039/b603379b
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发表时间：
2006-01-01
期刊：
影响因子：
6.1
通讯作者：
Dufva, M.
中科院分区：
文献类型：
--
作者：
Stangegaard, Michael;Petronis, S.;Dufva, M.
我们之前已经表明，具有介质灌注和集成热调节的聚合物（PMMA）芯片提供足够精确的热调节、pH控制和介质交换，以支持细胞生长数周。然而，目前还不清楚芯片中培养的细胞与培养瓶中培养的细胞有多相似。在当前的研究中，将芯片中培养的细胞的基因表达谱与培养瓶中培养的细胞的基因表达谱进行了比较。结果表明，与细胞培养瓶相比，在细胞培养芯片中生长的细胞中只有两种基因表达不同。该细胞培养芯片无需进一步修饰即可支持另外两种细胞系的细胞生长。在细胞的光学记录期间来自显微镜灯的光是唯一确定的外部因素，这可能对细胞存活产生负面影响。然而，低等级光暴露与光学记录以及细胞活力相容。这些结果有力地表明，与在黑暗和CO2条件培养箱中培养的培养瓶中培养的细胞相比，可以构建允许在线光学记录细胞事件而不影响细胞培养条件的细胞培养芯片。
We have previously shown that a polymeric (PMMA) chip with medium perfusion and integrated heat regulation provides sufficiently precise heat regulation, pH-control and medium exchange to support cell growth for weeks. However, it was unclear how closely the cells cultured in the chip resembled cells cultured in the culture flask. In the current study, gene expression profiles of cells cultured in the chip were compared with gene expression profiles of cells cultured in culture flasks. The results showed that there were only two genes that were differently expressed in cells grown in the cell culture chip compared to cell culture flasks. The cell culture chip could without further modification support cell growth of two other cell lines. Light coming from the microscope lamp during optical recordings of the cells was the only external factor identified, that could have a negative effect on cell survival. Low grade light exposure was however compatible with optical recordings as well as cell viability. These results strongly indicate that a cell culture chip could be constructed that allowed for on-line optical recording of cellular events without affecting the cell culturing condition compared to cell cultured in culture flasks incubated in a dark and CO2 conditioned incubator.