Reconstitution reveals the functional core of mammalian eIF3
Reconstitution reveals the functional core of mammalian eIF3
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DOI：
10.1038/sj.emboj.7601765
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发表时间：
2007-07-25
期刊：
影响因子：
11.4
通讯作者：
Imataka, Hiroaki
中科院分区：
文献类型：
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作者：
Masutani, Mamiko;Sonenberg, Nahum;Imataka, Hiroaki
真核翻译起始因子（eIF）3是哺乳动物细胞中最大的eIF（类似于650 kDa），由10-13个不同的多肽亚基组成。为了了解每个亚基的作用，我们成功地重建了由11个亚基组成的人eIF 3复合物，该复合物促进了40 S核糖体亚基向mRNA的募集。引人注目的是，eIF 3g和eIF 3 i亚基，这是进化上保守的人和酵母酿酒酵母之间的活性哺乳动物eIF 3复合物的形成是必需的。广泛的缺失分析表明，三个进化上保守的亚基（eIF 3a，eIF 3b和eIF 3c）和三个非保守的亚基（eIF 3e，eIF 3f和eIF 3 h）构成哺乳动物eIF 3的功能核心。
Eukaryotic translation initiation factor (eIF) 3 is the largest eIF (similar to 650 kDa), consisting of 10-13 different polypeptide subunits in mammalian cells. To understand the role of each subunit, we successfully reconstituted a human eIF3 complex consisting of 11 subunits that promoted the recruitment of the 40S ribosomal subunit to mRNA. Strikingly, the eIF3g and eIF3i subunits, which are evolutionarily conserved between human and the yeast Saccharomyces cerevisiae are dispensable for active mammalian eIF3 complex formation. Extensive deletion analyses suggest that three evolutionarily conserved subunits (eIF3a, eIF3b, and eIF3c) and three non-conserved subunits (eIF3e, eIF3f, and eIF3h) comprise the functional core of mammalian eIF3.