Production of fumaric acid by immobilized Rhizopus arrhizus on net.
Production of fumaric acid by immobilized Rhizopus arrhizus on net.
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DOI：
10.1016/j.biortech.2012.12.148
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发表时间：
2013-03
影响因子：
11.4
通讯作者：
Chunbo Gu;Yuqing Zhou;Luo Liu;T. Tan;Li Deng
中科院分区：
文献类型：
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作者：
Chunbo Gu;Yuqing Zhou;Luo Liu;T. Tan;Li Deng
采用网架固定化法对胡马酸发酵进行了研究。网的大表面固定了足够的丝状菌丝，迅速产生富马酸。为提高发酵性能，优化净粒大小和孢子浓度，最终选择净粒大小150cm2，孢子浓度0.5×106per ml。与自由细胞发酵相比，富马酸产量持平（32.03 g/L vs. 31.23g/L），但发酵时间缩短了83.3% （24 h vs. 144h）。
An immobilization method using net was developed for fumaric acid fermentation by Rhizopus arrhizus RH-07-13. The large surface of the net immobilized enough filamentous mycelia which produced fumaric acid rapidly. Net size and spore concentration were optimized to enhance fermentation performance and 150cm2of net size, 0.5×106per ml of spore concentration were selected finally. Compared to free-cell fermentation, fumaric acid production was flat (32.03 vs. 31.23g/L) but fermentation time reduced 83.3% (24 vs. 144h).