Polysomes are associated with microtubules in fertilized eggs of Chinese pine (Pinus tabulaeformis)
Polysomes are associated with microtubules in fertilized eggs of Chinese pine (Pinus tabulaeformis)
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多聚体与油松受精卵中的微管有关
DOI：
10.1007/s00709-005-0150-2
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发表时间：
2006-05-01
期刊：
影响因子：
2.9
通讯作者：
Watkins, Simon C.
中科院分区：
文献类型：
--
作者：
Han, Yuzhen;Yu, Jingjuan;Watkins, Simon C.
免疫金标记的黄松卵细胞受精前后的透射电镜显示，从受精到二核胚胎阶段，多聚体与微管（MTs）相关。受精前，卵的细胞质中随机分布着大小形状各异的核糖体聚集体。在这一阶段，单个mt或集群被观察到没有多体。受精后，所有的多聚体都附着在mt上，这种结合一直持续到极化胚胎的形成。此后，多聚体扩散到细胞质中，在胚胎中未观察到多聚体与mt的关联。一些多聚体附着在mt的一端，而另一些似乎沿着它们的整个长度形成接触。在发育的任何阶段都没有观察到多体与微丝的结合。多体与mt的结合可能为该植物早期胚胎发育中mt依赖的mRNA定位提供了一种机制。
Transmission electron microscopy of immunogold-labeled Chinese pine egg cells before and after fertilization revealed that polysomes are associated with microtubules (MTs) from fertilization to the 2-nucleate embryo stage. Ribosome aggregates of various size and shape were randomly distributed in the cytoplasm of the eggs before fertilization. Single MTs or clusters were observed to be free of polysomes at this stage. Upon fertilization, all polysomes were attached to MTs, and this association persisted until the formation of the polarized embryo. Thereafter, the polysomes spread into the cytoplasm and no polysome-MT association was observed in the embryo. Some of the polysomes were attached to one end of the MTs, while others appeared to form contacts along their entire length. No polysome-microfilament association was observed at any stage of the development. The polysome-MT association may provide a mechanism for MT-dependent mRNA localization in early embryo development of this plant.