Quantitation of time- and frequency-resolved optical spectra for the determination of tissue oxygenation.
Quantitation of time- and frequency-resolved optical spectra for the determination of tissue oxygenation.
复制标题
用于确定组织氧合的时间和频率分辨光谱的定量。
DOI：
10.1016/0003-2697(91)90339-u
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发表时间：
1991
影响因子：
2.9
通讯作者：
Maris,M
中科院分区：
文献类型：
--
作者：
Sevick,EM;Chance,B;Leigh,J;Nioka,S;Maris,M
近红外时间和频率分辨组织光谱技术的最新发展，探测组织氧合和组织氧合动力学，导致需要进一步定量的光谱信号。在本文中，我们简要回顾了在时域和频域中监测的强散射介质中的光传输理论，并使用该理论开发了从使用时间分辨光谱获得的光子衰减率（log R_t）和从频率分辨相位调制光谱获得的相移（θ）定量血红蛋白饱和度的算法。为了测试这些光学参数之间的关系，我们研究了在模拟组织的光学特性的模型系统中作为氧合的函数的对数R λ t和θ的行为。我们的研究结果表明，在不同的血红蛋白浓度的脱氧可以监测与光子衰减动力学的变化，在时间分辨测量中的Δ Δ log R Δ t，和与相移的变化，Δθ，在频率分辨技术。用这两种技术获得的成人大脑的光谱显示出从模型系统中识别出的相似特征。
The recent development of near-infrared time- and frequency-resolved tissue spectroscopy techniques to probe tissue oxygenation and tissue oxygenation kinetics has led to the need for further quantitation of spectroscopic signals. In this paper, we briefly review the theory of light transport in strongly scattering media as monitored in the time and frequency domains, and use this theory to develop algorithms for quantitation of hemoglobin saturation from the photon decay rate (∂ log R ∂t ) obtained using time-resolved spectroscopy, and from the phase-shift (θ) obtained from frequency-resolved, phase-modulated spectroscopy. To test the relationship of these optical parameters, we studied the behavior of ∂ log R ∂t and θ as a function of oxygenation in model systems which mimicked the optical properties of tissue. Our results show that deoxygenation at varying hemoglobin concentrations can be monitored with the change in the photon decay kinetics, Δ∂ log R ∂t in the time-resolved measurements, and with the change in phase-shift, Δθ, in the frequency-resolved technique. Optical spectra of the adult human brain obtained with these two techniques show similar characteristics identified from the model systems.