Detection by RNA blot hybridization of RNA sequences homologous to the BglII-N fragment of herpes simplex virus type 2 DNA.
Detection by RNA blot hybridization of RNA sequences homologous to the BglII-N fragment of herpes simplex virus type 2 DNA.
复制标题
通过 RNA 印迹杂交检测与 2 型单纯疱疹病毒 DNA 的 BglII-N 片段同源的 RNA 序列。
DOI：
10.1128/jvi.44.3.1092-1096.1982
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发表时间：
1982
影响因子：
5.4
通讯作者：
Rapp,F
中科院分区：
文献类型：
--
作者：
Jenkins,FJ;Howett,MK;Spector,DJ;Rapp,F
RNA species, extracted at the time of peak synthesis of the alpha, beta, and gamma classes of herpes simplex virus polypeptides from lytically infected Vero cells, were examined for homology to the BglII-N fragment (map units 0.58 to 0.63) of herpes simplex virus type 2 DNA. By using northern blot analysis, two major and several minor polyadenylated RNA species showed homology to the BglII-N fragment at times corresponding to the maximum synthesis of the beta (7 h postinfection) and gamma (12 h postinfection) herpes simplex virus polypeptides. No alpha RNA homologous to the BglII-N fragment was detected.
