Seasonal fluctuation in the occurrence of Xylella fastidiosa in root and stem extracts from citrus with blight
Seasonal fluctuation in the occurrence of Xylella fastidiosa in root and stem extracts from citrus with blight
复制标题
枯萎病柑橘根和茎提取物中木霉的发生率随季节变化
DOI：
10.1094/pd-75-0145
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发表时间：
1991
期刊：
影响因子：
4.5
通讯作者：
C. M. Thompson
中科院分区：
文献类型：
--
作者：
D. Hopkins;F. W. Bistline;L. Russo;C. M. Thompson
在佛罗里达州南部两个枯萎病严重的柑橘园中，对出现枯萎病症状的成熟树木进行了取样，并通过酶联免疫吸附测定（ELISA）检测了木霉的存在情况。每月从每个树林中随机选择 12 棵患有枯萎病的树木，获取根和茎提取物。在这两个树林中，每年有两个 X. fastidiosa 的检测高峰期：仲夏（6 月至 8 月）和仲冬（12 月至 2 月）。四月、五月或十月的树木提取物中未检测到细菌。根和茎提取物的季节模式和检测频率相似。 ELISA 检测呈阳性的样品中，可以从不到 10% 的样品中培养出 X. fastidiosa 菌株 (.)
In two citrus groves in southern Florida where blight is severe, mature trees with blight symptoms were sampled and tested for the presence of Xylella fastidiosa by means of enzyme-linked immunosorbent assay (ELISA). Root and stem extracts were obtained monthly from 12 randomly selected trees with blight from each grove. In both groves, there were two yearly peak periods of detection of X. fastidiosa, midsummer (June-August) and midwinter (December-February). Bacteria were not detected in tree extracts in April, May, or October. The seasonal pattern and frequency of detection were similar for both root and stem extracts. Strains of X. fastidiosa could be cultured from fewer than 10% of the samples that were positive by ELISA (.)