Expression of CD44v6 and integrin-β1 for the prognosis evaluation of pancreatic cancer patients after cryosurgery.
Expression of CD44v6 and integrin-β1 for the prognosis evaluation of pancreatic cancer patients after cryosurgery.
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DOI：
10.1186/1746-1596-8-146
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发表时间：
2013-09-02
影响因子：
2.6
通讯作者：
Xu K
中科院分区：
文献类型：
--
作者：
Zhou G;Chiu D;Qin D;Niu L;Cai J;He L;Tan D;Xu K
许多先前的研究表明，细胞粘附分子CD44v6和整合素-β1已被广泛研究作为各种癌症的潜在预后标志物。然而，PC的数据是稀缺的。我们现在通过三重实时RT-PCR检测CD44v6和整合素-β 1mrna在PBMC中的表达，并通过ELISA检测血浆中的蛋白表达。所有标本采集自54例接受冷冻手术治疗的PC患者和20例健康个体（对照组）。与对照组相比，患者CD44v6、整合素-β1 mRNA及蛋白表达水平均显著升高（P<0.05）。CD44v6 mRNA和蛋白的高表达与临床分期、肿瘤分化、LNM、肝转移及中位DFS降低显著相关（P<0.05），整合素β1 mRNA和蛋白的高表达与临床分期、LNM、肝转移及中位DFS降低显著相关（P<0.05）。临床分期、LNM、肝转移、CD44v6 mRNA及蛋白表达是影响PC患者生存的独立预测因素（P<0.05）。冷冻后3个月患者CD44v6、整合素-β1 mRNA及蛋白表达水平均显著降低（P<0.05）。冷冻术后3个月患者与对照组CD44v6 mRNA及蛋白表达差异无统计学意义（P < 0.05）。血液中CD44v6和整合素-β1 mRNA和蛋白的表达可作为肝癌发生转移的生物标志物和预后指标。它们可能成为评估冷冻手术后PC患者预后的有用预测因子。本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/4035308681009006。
Many previous studies demonstrated that cell adhesion molecules CD44v6 and integrin-β1 had been extensively investigated as potential prognostic markers of various cancers. However, data in PC are scarce. We now investigate CD44v6 and integrin-β1 mRNA expression in PBMC by a triplex real-time RT-PCR assay and protein expression in plasma by ELISA. All specimens were collected from 54 PC patients who received the treatment of cryosurgery as well as 20 healthy individuals (control). The mRNA and protein expression levels of CD44v6 and integrin-β1 in patients were significantly increased compared with control group (P<0.05). The high CD44v6 mRNA and protein expression were significantly correlated with clinical stage, tumor differentiation, LNM, liver metastasis and decreased median DFS (P<0.05), while the high integrin-β1 mRNA and protein expression were significantly correlated with clinical stage, LNM, liver metastasis and decreased median DFS (P<0.05). Clinical stage, LNM, liver metastasis, CD44v6 mRNA and protein expression were the independent predictors of survival in PC patients (P<0.05). Moreover, CD44v6 and integrin-β1 mRNA and protein expression levels were significantly decreased in patients in 3 months after cryosurgery (P<0.05). No significant difference was found in CD44v6 mRNA and protein expression between patients in 3 months after cryosurgery and control group (P>0.05). CD44v6 and integrin-β1 mRNA and protein expression in blood may serve as biomarkers for the development and metastasis of PC, and as prognostic indicators for PC. They may become useful predictors in assessing outcome of PC patients after cryosurgery. The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4035308681009006.
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影响因子：
2.7
作者：
Baust, JG;Gage, AA;Van Buskirk, R
通讯作者：
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影响因子：
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