Development of the anterior commissure in the opossum: midline extracellular space and glia coincide with early axon decussation.
Development of the anterior commissure in the opossum: midline extracellular space and glia coincide with early axon decussation.
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DOI：
10.1002/(sici)1097-4695(199704)32:4
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发表时间：
1997-04
期刊：
影响因子：
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通讯作者：
D. Cummings;D. Malun;P. Brunjes
中科院分区：
文献类型：
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作者：
D. Cummings;D. Malun;P. Brunjes
虽然前连合已被证明是前脑中信息传递的重要途径，但对其解剖学发育的了解相对较少。神经胶质基质和细胞外间隙与其他大纤维束的成熟有关，如胼胝体和离视网膜途径。本研究探讨了在连合成熟的早期阶段，在灰色短尾负鼠，Monodelphis南极。Monodelphis的后代在短短的14天妊娠后出生，并且与大鼠和小鼠不同，前连合完全在出生后时期发育。采用了一些技术：碳菁染料Dil被用来标记该地区的早期轴突，半薄塑料切片被用来检查细胞外环境的发展连合，和胶质细胞酸性蛋白（GFAP）的免疫细胞化学被用来表征胶质成分。结果表明，穿过中线的第一连合纤维通过一个大的细胞外空间的区域，并可能使用GFAP免疫反应阳性细胞和过程作为指导，在他们的中线交叉。
While the anterior commissure has been shown to be an important route of information transfer in the forebrain, relatively little is known about its anatomical development. Glial substrates and extracellular spaces have been associated with the maturation of other large-fiber tracts, such as the corpus callosum and retinofugal pathway. The present study examined early stages in the maturation of the commissure in the gray short-tailed opossum, Monodelphis domestica. Monodelphis offspring are born after a short 14-day gestation, and, unlike in rats and mice, the anterior commissure develops entirely during the postnatal period. A number of techniques were employed: the carbocyanine dye Dil was used to label early axons in the region, semithin plastic sections were used to examine the extracellular environment of the developing commissure, and immunocytochemistry for glial fibrillary acidic protein (GFAP) was used to characterize glial components. Results suggest that the first commissural fibers that cross the midline pass through a region of large extracellular spaces and may use GFAP-immunoreactive cells and processes as guides during their midline decussation.