Association of the Nramp1 gene polymorphisms and clinical forms in patients with tuberculosis
Association of the Nramp1 gene polymorphisms and clinical forms in patients with tuberculosis
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DOI：
10.4149/bll_2012_148
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发表时间：
2012-01-01
影响因子：
1.5
通讯作者：
Seydaoglu, G.
中科院分区：
文献类型：
--
作者：
Hanta, I;Tastemir-Korkmaz, D.;Seydaoglu, G.
背景：最近的研究报道了Nramp1多态性可能在不同人群的结核病发展中起重要作用。在这项研究中，我们旨在确定Nramp1多态性在我们的结核病患者群体中。方法：我们招募了127例活动性肺结核患者和116例年龄和性别相近的健康成人。采集外周血样本以确定Nramp1多态性。采用聚合酶链反应(PCR) -限制性片段长度多态性（RFLP）技术，对Nramp1基因在D543N和INT4位点的多态性进行了分析。结果：我们发现Nramp1在D543N区域的多态性（GA等位基因OR: 0.44, 95%CI: 0.09-2.06）不是结核病的危险因素。此外，我们无法在INT4区域检测到Nramp1多态性（GC等位基因OR: 0.97, 95%CI: 0.55-1.72， CC等位基因OR: 0.90, 95%CI: 0.21-3.77）。结论：本研究结果不支持Nramp1在D543和INT4区域可能影响杆菌生长和空洞结核进展的假设，而不是对M. tuberculosis感染的易感性。需要进一步的研究来阐明Nramp1变异在结核病发病机制中的作用（表3，参考文献29）。PDF全文www.elis.sk。
Background: Recent studies have reported that Nramp1 polymorphisms might have an important role in the development of tuberculosis in various populations. In this study, we aimed to determine Nramp1 polymorphisms in our patients with tuberculosis population.Methods: We enrolled 127 patients with active tuberculosis and 116 healthy adults with similar age and gender. Peripheral blood samples were taken for determining the Nramp1 polymorphisms. By using Polymerase Chain Reaction (PCR) - Restriction Fragment Length Polymorphisms (RFLP) technique, we evaluated the polymorphisms of Nramp1 at the regions of D543N and INT4.Results: We found that the Nramp1 polymorphisms at the region of D543N (OR: 0.44, 95%CI: 0.09-2.06 for GA allele) were not a risk factor for tuberculosis. Furthermore, we could not able to detect Nramp1 polymorphism at the regions of INT4 (OR: 0.97, 95%CI: 0.55-1.72 for GC allele and OR: 0.90, 95%CI: 0.21-3.77 for CC allele).Conclusion: The findings of the present study do not support the hypothesis that Nramp1 at the regions of D543 and INT4 might play a role in influencing the growth of bacilli and progression of cavitary tuberculosis rather than susceptibility to M. tuberculosis infection. Future studies are needed to elucidate the role of Nramp1 variants in the pathogenesis of tuberculosis (Tab. 3, Ref. 29). Full Text in PDF www.elis.sk.