HYDROGEN PEROXIDE-INDUCIBLE PROTEINS IN SALMONELLA-TYPHIMURIUM OVERLAP WITH HEAT-SHOCK AND OTHER STRESS PROTEINS
HYDROGEN PEROXIDE-INDUCIBLE PROTEINS IN SALMONELLA-TYPHIMURIUM OVERLAP WITH HEAT-SHOCK AND OTHER STRESS PROTEINS
复制标题
DOI：
10.1073/pnas.83.21.8059
复制
发表时间：
1986-11-01
影响因子：
11.1
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
MORGAN, RW;CHRISTMAN, MF;AMES, BN
过氧化氢处理诱导鼠伤寒沙门菌30种蛋白的合成。其中五种蛋白质也会被热休克诱导，包括高度保守的DnaK蛋白。热休克诱导这五种蛋白中的一种依赖于氧r，氧r是过氧化氢诱导基因的正调节因子，而热休克诱导其他四种蛋白则不依赖于氧r。30种过氧化氢诱导蛋白中的5种已被鉴定，其结构基因已被绘制。其他胁迫如萘啶酸、乙醇或异丙苯氢过氧化处理也可诱导30种过氧化氢诱导蛋白的亚群以及其他蛋白质。过氧化氢诱导的蛋白质与有氧作用诱导的蛋白质有很大的不同。此外，过氧化氢酶（katG）基因的表达在mRNA水平上受到氧还原的调控。
Hydrogen peroxide treatment induces the synthesis of 30 proteins in Salmonella typhimurium. Five of these proteins are also induced by heat shock, including the highly conserved DnaK protein. The induction of one of these five proteins by heat shock is dependent on oxyR, a positive regulator of hydrogen peroxide-inducible genes, while the induction of the other four by heat shock is oxyR independent. Five of the 30 hydrogen peroxide-inducible proteins have been identified, and their structural genes have been mapped. Other stresses such as nalidixic acid, ethanol, or cumene hydroperoxide treatment also induce subsets of the 30 hydrogen peroxide-inducible proteins as well as additional proteins. Hydrogen peroxide-inducible proteins are shown to be largely different from those proteins induced by aerobiosis. In addition, the expression of the katG (catalase) gene is shown to be regulated by oxyR at the level of mRNA.