A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping.
A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping.
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DOI：
10.1016/j.cell.2014.11.021
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发表时间：
2014-12-18
期刊：
影响因子：
64.5
通讯作者：
Aiden EL
中科院分区：
文献类型：
--
作者：
Rao SS;Huntley MH;Durand NC;Stamenova EK;Bochkov ID;Robinson JT;Sanborn AL;Machol I;Omer AD;Lander ES;Aiden EL
我们使用原位Hi-C来探测基因组的三维结构，构建九种细胞类型的单倍体和二倍体图谱。在人类淋巴母细胞中的denatrium包含49亿个触点，实现了1-脱氢酶分辨率。我们发现，基因组被划分为局部域，这是与不同的组蛋白标记模式和隔离成六个亚室。我们识别了~ 10，000个循环。这些环经常连接启动子和增强子，与基因激活相关，并在细胞类型和物种中显示出保守性。环锚通常出现在结构域边界并结合CTCF。环锚定处的CTCF位点主要（>90%）以会聚取向出现，其中不对称基序彼此“面对”。失活的X染色体分裂成两个巨大的结构域，并含有锚定在CTCF结合重复序列上的大环。环锚通常发生在结构域边界处，并以会聚取向结合CTCF，其中不对称基序彼此“面对”。在失活的X染色体上，大的印记环锚定在CTCF结合重复序列处。环在细胞类型和物种中是保守的。
We use in situ Hi-C to probe the three-dimensional architecture of genomes, constructing haploid and diploid maps of nine cell types. The densest, in human lymphoblastoid cells, contains 4.9 billion contacts, achieving 1-kilobase resolution. We find that genomes are partitioned into local domains, which are associated with distinct patterns of histone marks and segregate into six subcompartments. We identify ~10,000 loops. These loops frequently link promoters and enhancers, correlate with gene activation, and show conservation across cell types and species. Loop anchors typically occur at domain boundaries and bind CTCF. CTCF sites at loop anchors occur predominantly (>90%) in a convergent orientation, with the asymmetric motifs ‘facing’ one another. The inactive X-chromosome splits into two massive domains and contains large loops anchored at CTCF-binding repeats. Loop anchors typically occur at domain boundaries and bind CTCF in a convergent orientation, with the asymmetric motifs ‘facing’ one another. On the inactive X-chromosome, large imprinted loops are anchored at CTCF-binding repeats. Loops are conserved across cell types and species.
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影响因子：
64.8
作者：
Jin, Fulai;Li, Yan;Dixon, Jesse R.;Selvaraj, Siddarth;Ye, Zhen;Lee, Ah Young;Yen, Chia-An;Schmitt, Anthony D.;Espinoza, Celso A.;Ren, Bing
通讯作者：
Ren, Bing
影响因子：
46.9
作者：
通讯作者：
--
来自1,092个人基因组的遗传变异的综合图。
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
3.5
作者：
Horakova, Andrea H.;Moseley, Shawn C.;Chadwick, Brian P.
通讯作者：
Chadwick, Brian P.
影响因子：
64.5
作者：
MUKHERJEE, S;ERICKSON, H;BASTIA, D
通讯作者：
BASTIA, D