Rhodopsin phosphorylation and its role in photoreceptor function
Rhodopsin phosphorylation and its role in photoreceptor function
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DOI：
10.1016/s0042-6989(97)00459-8
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发表时间：
1998-05-01
期刊：
影响因子：
1.8
通讯作者：
Niemi, GA
中科院分区：
文献类型：
--
作者：
Hurley, JB;Spencer, M;Niemi, GA
25 年前首次描述了视紫红质的光刺激磷酸化。本文回顾了在（i）了解磷酸化和去磷酸化视紫红质的酶的性质（ii）识别视紫红质磷酸化位点和（iii）了解视紫红质磷酸化的生理重要性方面所取得的进展。与视紫红质磷酸化相关的许多重要问题仍未得到解答，需要新的策略和方法来解决 Ca2+ 和恢复蛋白的作用等问题。我们提出了一种这样的方法，使用质谱法来定量完整小鼠视网膜中的视紫红质磷酸化。 (C) 1998 年由爱思唯尔科学有限公司出版。保留所有权利。
Light-stimulated phosphorylation of rhodopsin was first described 25 years ago. This paper reviews the progress that has been made towards (i) understanding the nature of the enzymes that phosphorylate and dephosphorylate rhodopsin (ii) identifying the sites of phosphorylation on rhodopsin and (iii) understanding the physiological importance of rhodopsin phosphorylation. Many important questions related to rhodopsin phosphorylation remain unanswered and new strategies and methods are needed to address issues such as the roles of Ca2+ and recoverin. We present one such method that uses mass spectrometry to quantitate rhodopsin phosphorylation in intact mouse retinas. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.