Reversible Chemosensor for Bioimaging and Biosensing of Zn(II) and hpH in Cells, Larval Zebrafish, and Plants with Dual-Channel Fluorescence Signals
Reversible Chemosensor for Bioimaging and Biosensing of Zn(II) and hpH in Cells, Larval Zebrafish, and Plants with Dual-Channel Fluorescence Signals
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DOI：
10.1021/acs.inorgchem.0c03456
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发表时间：
2021-03-05
影响因子：
4.6
通讯作者：
Shen, Jianliang
中科院分区：
文献类型：
--
作者：
He, Xiaojun;Ding, Feng;Shen, Jianliang
锌/Zn（II）是人体必需的微量元素，是维持机体正常生长、发育和代谢的重要物质。Zn（II）过量或缺乏都会引起人体代谢异常，导致一系列疾病。此外，生物系统具有复杂的稳态系统，特别是苛刻的pH（OH-）环境。因此，研究Zn（II）和OH-水平的变化在临床，医学和环境测试中非常重要。然而，缺乏实用和方便的荧光成像工具限制了Zn（II）和OH-在生物系统中的跟踪。在这项工作中，一个精心设计的双通道荧光信号响应化学传感器（DACH-fhba）组装的选择性传感Zn（II）和OH-在生物系统中使用荧光打开策略。在遇到Zn（II）时，化学传感器发射蓝色荧光信号（455 nm）。同时，随着OH-的加入，明亮的绿色荧光信号（530 nm）也随之增强.利用DACH-fhba的蓝/绿色双重荧光响应，该传感器具有较高的稳定性和可逆性。值得注意的是，生物成像显示DACH-fhba成功地追踪了活细胞、斑马鱼幼虫和植物中的Zn（II）和OH-。进一步的结果表明，DACH-fhba可用于实现生物体中Zn（II）和OH-的视觉检测。总之，这项工作是有益的Zn（II）和OH-在生物体中的监测和促进了解Zn（II）和OH-在生物系统中的功能。
Zinc/Zn(II) is an essential trace element for humans and acts as an important substance that maintains the normal growth, development, and metabolism of the body. Excess or deficient Zn(II) can cause abnormal metabolism in the human body, leading to a series of diseases. Moreover, biosystems have complex homeostasis systems, especially harsh pH (OH-) environments. Thus, investigating the variation in the levels of Zn(II) and OH- is extremely important in clinical, medical, and environmental testing. Nevertheless, the lack of practical and convenient fluorescence imaging tools limits the tracing of Zn(II) and OH- in biosystems. In this work, a well-designed dual-channel fluorescent signal response chemosensor (DACH-fhba) was assembled for selective sensing of Zn(II) and OH- in the biosystem using a fluorescence turn-on strategy. On encountering Zn(II), the chemosensor emitted a blue fluorescence signal (455 nm). Meanwhile, the bright green fluorescence signal (530 nm) increased with OH- addition simultaneously. With the blue/green dual fluorescence response of DACH-fhba, the sensor exhibited high stability and reversibility. Notably, the bioimaging revealed that DACH-fhba successfully tracked Zn(II) and OH- in live cells, larval zebrafish, and plants. Further results implied that DACH-fhba can be used to achieve visual detection of Zn(II) and OH- in organisms. Altogether, this work is conducive to the monitoring of Zn(II) and OH- in organisms and promotes the understanding of the function of Zn(II) and OH- in biosystems.