Genome-wide location of yeast RNA polymerase III transcription machinery
Genome-wide location of yeast RNA polymerase III transcription machinery
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DOI：
10.1093/emboj/cdg466
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发表时间：
2003-09-15
期刊：
影响因子：
11.4
通讯作者：
Lefebvre, O
中科院分区：
文献类型：
--
作者：
Harismendy, O;Gendrel, CG;Lefebvre, O
RNA聚合酶III(Pol III)转录一大组编码tRNAs、5S rRNA、U6 SnRNA或RPR1 RNA等未翻译小RNA的基因。为了获得III类(Pol III-转录)基因的全局视图，对Pol III、TFIIIC和TFIIIB的基本成分在酵母基因组中的分布进行了定位。在生长活跃期间，TFIIIC、TFIIIB和POL III以大多数III类基因和少数额外的基因为靶点，表明它们在转录上是活跃的。编码snoRNA的SNR52被鉴定为一个新的III类基因。在生长后期，TFIIIC仍然与大多数III类基因结合，而Pol III和TFIIIB的招募受到下调。这项研究确定了酵母中III类基因数量的合理上限，并指出在POL III和TFIIIB招募水平上存在全球调控。
RNA polymerase III (Pol III) transcribes a large set of genes encoding small untranslated RNAs like tRNAs, 5S rRNA, U6 snRNA or RPR1 RNA. To get a global view of class III (Pol III-transcribed) genes, the distribution of essential components of Pol III, TFIIIC and TFIIIB was mapped across the yeast genome. During active growth, most class III genes and few additional loci were targeted by TFIIIC, TFIIIB and Pol III, indicating that they were transcriptionally active. SNR52, which encodes a snoRNA, was identified as a new class III gene. During the late growth phase, TFIIIC remained bound to most class III genes while the recruitment of Pol III and, to a lesser extent, of TFIIIB was down regulated. This study fixes a reasonable upper bound to the number of class III genes in yeast and points to a global regulation at the level of Pol III and TFIIIB recruitment.