Glutathione transferases.
Glutathione transferases.
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DOI：
10.1199/tab.0131
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Edwards, Robert
中科院分区：
文献类型：
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作者：
Dixon, David P;Edwards, Robert
除微粒体外，55 种拟南芥谷胱甘肽转移酶 (GST) 是一组单系可溶性酶，可分为 phi、tau、theta、zeta、lambda、脱氢抗坏血酸还原酶 (DHAR) 和 TCHQD 类。数量众多的 phi 和 tau 类通常是高度应激诱导的，并且经常出现在蛋白质组学和转录组学研究中。尽管对其外源物质解毒活性进行了大量研究，但它们的自然作用尚不清楚，但可能在防御相关的次生代谢中发挥作用。较小的 DHAR 和 lambda 类可能是谷胱甘肽依赖性还原酶，zeta 类在酪氨酸分解代谢中发挥作用，theta 类在氧化脂质解毒中发挥推定作用。这篇综述描述了 GST 功能作用的证据以及这些酶执行多种功能的潜力，这些功能在许多情况下不是“谷胱甘肽转移酶”活性。除了生化数据外，还包括蛋白质组学和转录组学研究的表达数据，以及亚细胞定位实验和功能基因组研究的结果。
The 55 Arabidopsis glutathione transferases (GSTs) are, with one microsomal exception, a monophyletic group of soluble enzymes that can be divided into phi, tau, theta, zeta, lambda, dehydroascorbate reductase (DHAR) and TCHQD classes. The populous phi and tau classes are often highly stress inducible and regularly crop up in proteomic and transcriptomic studies. Despite much study on their xenobiotic-detoxifying activities their natural roles are unclear, although roles in defence-related secondary metabolism are likely. The smaller DHAR and lambda classes are likely glutathione-dependent reductases, the zeta class functions in tyrosine catabolism and the theta class has a putative role in detoxifying oxidised lipids. This review describes the evidence for the functional roles of GSTs and the potential for these enzymes to perform diverse functions that in many cases are not "glutathione transferase" activities. As well as biochemical data, expression data from proteomic and transcriptomic studies are included, along with subcellular localisation experiments and the results of functional genomic studies.