Molecular hydrogen production by nitrogenase of Rhodobacter sphaeroides and by Fe-only hydrogenase of Rhodospirillum rubrum
Molecular hydrogen production by nitrogenase of Rhodobacter sphaeroides and by Fe-only hydrogenase of Rhodospirillum rubrum
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DOI：
10.1016/j.ijhydene.2007.09.044
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发表时间：
2008-03-01
影响因子：
7.2
通讯作者：
Lee, Jeong K.
中科院分区：
文献类型：
--
作者：
Kim, Eui-Jin;Lee, Moon-Kyu;Lee, Jeong K.
球形红杆菌的两个PII类蛋白GlnB和GlnK的编码基因从染色体上中断，这两个蛋白在氨离子存在下对固氮酶的表达起着关键作用。与其亲本相比，glnB-glnK突变体对固氮酶的氨离子介导的抑制作用较小，这导致在此条件下突变体积累了更多的H-2。红色红螺菌通过固氮酶和氢酶产生H-2。在光营养条件下，含有含有编码自身铁-唯一氢酶的HYDC的重组pRK415的红色红假单胞菌在光营养条件下H-2的积累增加了一倍，而在没有丙酮酸的情况下没有观察到。含有编码乙酰丁酸梭菌铁氢酶的重组pRK415的红色葡萄球菌也是如此。因此，只有铁的氢酶需要丙酮酸作为电子供体来产生H-2。(C)2007年国际氢能协会。爱思唯尔有限公司出版。保留所有权利。
The genes coding for two PII-like proteins, GlnB and GlnK, which play key roles in repressing the nitrogenase expression in the presence of ammonium ion, were interrupted from the chromosome of Rhodobacter sphaeroides. The glnB-glnK mutant exhibits the less ammonium ion-mediated repression for nitrogenase compared with its parental strain, which results in more H-2 accumulation by the mutant under the conditions. Rhodospirillum rubrum produces H-2 by both nitrogenase and hydrogenase. R. rubrum containing the recombinant pRK415 with an insert of hydC coding for its own Fe-only hydrogenase showed twofold higher accumulation of H-2 in the presence of pyruvate under photcheterotrophic conditions, which was not observed in the absence of pyruvate. The same was true with R. rubrum containing the recombinant pRK415 cloned with hydA coding for Fe-only hydrogenase of Clostridium acetobutylicum. Thus, Fe-only hydrogenase requires pyruvate as an electron donor for the production of H-2. (c) 2007 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.