Resveratrol restores lysophosphatidylcholine‐induced loss of endothelium‐dependent relaxation in rat aorta tissue coinciding with inhibition of extracellular‐signal‐regulated protein kinase activation
Resveratrol restores lysophosphatidylcholine‐induced loss of endothelium‐dependent relaxation in rat aorta tissue coinciding with inhibition of extracellular‐signal‐regulated protein kinase activation
复制标题
白藜芦醇可恢复大鼠主动脉组织中溶血磷脂酰胆碱诱导的内皮依赖性松弛的丧失，同时抑制细胞外信号调节的蛋白激酶激活
作者：
Z. Min;L. Kang;Li Lin;Fan Jinghua;Song Junna;L. Baolin
为探讨白藜芦醇能否恢复溶血磷脂酰胆碱(LPC)所致的体外培养大鼠主动脉内皮依赖性舒张功能的丧失，本研究首先观察了白藜芦醇对EDR的影响。结果表明，白藜芦醇以浓度依赖的方式(1，10,100 μm)减弱LPC(10 μm)对内皮源性松弛因子和内皮衍生超极化因子的抑制作用。此外，白藜芦醇可抑制K+诱导的血管收缩，但对ACh(1 μm)诱导的内皮依赖松弛无明显影响。选择性ERK抑制剂PD 98059(30 μm)也有类似的趋势。当LPC(20 μm)作用于细胞后，eNOS和COX-1mRNA的表达下调，而iNOS和COX-2的表达被局部诱导。白藜芦醇和PD 98059成功地逆转了LPC对eNOS和COX-2表达的影响。白藜芦醇和PD 98059均可抑制LPC诱导的ERK激活。这些结果表明，白藜芦醇可以恢复LPC诱导的大鼠主动脉EDR的丢失，并通过抑制炎症样反应来保护内皮免受LPC诱导的损伤。版权所有©2010 John Wiley&Sons，Ltd.
To investigate whether resveratrol could restore the lysophosphatidylcholine (LPC)‐induced loss of endothelium‐dependent relaxation in in vitro cultured rat aorta tissue, first the effect of resveratrol on the loss of EDR was examined in this preparation. The results showed that resveratrol effectively attenuated the inhibition of LPC (10 μm) on both endothelium‐derived relaxing factor (EDRF) and endothelium‐derived hyperpolarizing factor (EDHF) in a concentration‐dependent manner (1, 10, 100 μm). In addition, resveratrol inhibited elevated K+‐induced vascular contracture, but had no significant effects on ACh (1 μm)‐induced endothelium‐dependent relaxation (EDR). A similar tendency was also observed with PD 98059 (30 μm), a selective inhibitor of ERK. When the cells were exposed to LPC (20 μm) the mRNA expression of eNOS and COX‐1 mRNA were down‐regulated, followed by a local induction of iNOS and COX‐2. Resveratrol and PD 98059 successfully reversed the effects of LPC on relative mRNA expressions. Both resveratrol and PD 98059 inhibited the activation of ERK induced by LPC. These findings demonstrate that resveratrol can restore the LPC‐induced loss of EDR in rat aorta and protect the endothelium against LPC‐induced injuries via the inhibition of the inflammation‐like response. Copyright © 2010 John Wiley & Sons, Ltd.
影响因子：
20.1
作者：
Campbell, WB;Gebremedhin, D;Harder, DR
通讯作者：
Harder, DR