Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii
Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii
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DOI：
10.2147/idr.s196575
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发表时间：
2019-01-01
影响因子：
3.9
通讯作者：
Goodarzi, Hamed
中科院分区：
文献类型：
--
作者：
Amin, Mansour;Navidifar, Tahereh;Goodarzi, Hamed
背景资料：金属β-内酰胺酶（MBL）通常编码在携带整合子的基因盒上，在鲍曼不动杆菌中容易传播。本研究旨在探讨MBL编码基因与多药耐药（MDR）A中1类和2类整合子的关系。方法：共收集85株非重复鲍曼不动杆菌。收集鲍曼不动杆菌分离株并评价bla（OXA-51）的扩增。用PCR方法检测MBL基因，包括bla（IMP）、bla（Vim）、bla（SIM）、bla（SPM）、bla（GIM）、bla（DIM）和bla（NDM），以及intI 1和intI 2。结果：在85株耐药菌株中，有77株为耐药菌株。此外，34个分离株仅intI 1，10个仅intI 2，15个同时具有intI 1和intI 2。在30株菌株中检测到MBL基因，其中以blaVIM基因最常见，其次为blaIMP、blaSPM和blaSIM。基因盒分析表明，1类整合子通常负责携带MBLs基因的转移。鲍曼不动杆菌是碳青霉烯类抗生素耐药的主要机制之一。因此，在临床实验室环境中开发用于MBL表型检测的廉价筛选方法是必不可少的。此外，我们的数据显示，1类整合子往往是负责传播的MBL基因之间的A。鲍曼不动杆菌分离株。
Background: Metallo-beta-Lactamases (MBL) are usually encoded on the gene cassettes harboring integrons and disseminated easily among Acinetobacter baumannii isolates. This study was aimed to investigate the association of the genes encoding MBL with the presence of class 1 and 2 integrons among multidrug-resistant (MDR) A. baumannii isolates.Methodology: A total of 85 non-duplicated A. baumannii isolates were collected and evaluated for the amplification of bla(OXA-51). The presence of genes encoding MBLs, including bla(IMP), bla(VIM), bla(SIM), bla(SPM), bla(GIM), bla(DIM) and bla(NDM), as well as intI 1 and intI 2 was evaluated by PCR. Also, the production of MBLs was screened phenotypically by the combination of EDTA and meropenem.Results: In this study, 77 out of 85 isolates were MDR. Also, 34 isolates had only intI 1, 10 had only intI 2 and 15 had both intI 1 and intI 2. The phenotypic detection of MBLs was found in 30 isolates, among which blaVIM was as the most common the gene encoding MBL followed by blaIMP, blaSPM and blaSIM. The gene cassettes analysis revealed that class 1 integron is often responsible for transferring the genes harboring MBLs.Conclusion: The production of MBLs among A. baumannii strains is one of the main mechanisms of resistance to carbapenems. Therefore, the development of inexpensive screening methods for the phenotypic detection of MBLs in clinical laboratories settings is essential. Also, our data revealed that the class 1 integron is often responsible for the dissemination of the MBL genes among A. baumannii isolates.