Chryseobacterium binzhouense sp. nov., isolated from activated sludge
Chryseobacterium binzhouense sp. nov., isolated from activated sludge
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滨州金杆菌
DOI：
10.1099/ijsem.0.003800
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发表时间：
2020
影响因子：
2.8
通讯作者：
Li Qiang
中科院分区：
文献类型：
--
作者：
Meng Dong;Liu Yu-Ling;Li Rui-Rui;Cu Peng-Fei;Fan Xiang-Yu;Huang Zhao-Song;Du Zong-Jun;Li Qiang
从山东省滨州市某污水处理厂的活性污泥中分离到一株金黄色、革兰氏阴性、好氧棒状、无鞭毛和不滑行的细菌，命名为菌株lm2T。生长条件为15-45℃（最适为30℃），NaCl浓度为0-5.0 % (w/v)（最适为0-2.0 %），pH为6.5-8.0（最适为7.0-7.5）。对其化学分类、表型和基因组性状进行了研究。基于16S rRNA基因序列的系统发育分析表明，菌株lm2t属于黄杆菌属，与棘球黄杆菌cc - czw010t序列相似性最高（97.1%）。基因组测序结果显示，其基因组大小为3 611 894 bp， G+C含量为34.9 mol%。菌株lm2TandC之间的平均核苷酸同源值和数字DNA-DNA杂交（dDDH）值。echinoideorumJCM 30470分别为87.8%和34.7%。呼吸道醌类主要为甲基萘醌-6 （MK-6）。主要脂肪酸为iso-C15: 0、iso-C17: 03-OH和iso-C17: 1ω9c，其极性脂质由磷脂酰乙醇胺（PE）、未识别的脂质（L1-5）和未识别的氨基脂质（l1 - 4）组成。在此基础上，菌株lm2被认为代表了一种新的黄杆菌属，并因此被命名为滨州红杆菌。11月被提议。型应变为lm2T（=KCTC 72529T=CCTCC AB2019126T）。
A golden-pigmented, Gram-strain-negative, aerobic, rod-shaped, non-flagellated and non-gliding bacterium, designated strain lm2T, was isolated from activated sludge obtained from a wastewater treatment plant in Binzhou (Shandong province, PR China). Growth occurred at 15–45°C (optimum, 30 °C), in the presence of 0–5.0 % (w/v) NaCl (optimum, 0–2.0 %) and at pH 6.5–8.0 (optimum, pH 7.0–7.5). The chemotaxonomic, phenotypic and genomic traits were investigated. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain lm2Tbelonged to the genusChryseobacterium, with highest sequence similarity toChryseobacterium echinoideorumCC-CZW010T(97.1 %). Genome sequencing revealed a genome size of 3 611 894 bp and a G+C content of 34.9 mol%. The average nucleotide identity value and the digital DNA–DNA hybridization (dDDH) value between strain lm2TandC. echinoideorumJCM 30470Twere 87.8 and 34.7 %, respectively. The major respiratory quinone was Menaquinone-6 (MK-6). The major fatty acids were iso-C15 : 0, iso-C17 : 03-OH and iso-C17 : 1ω9cand its polar lipids consisted of phosphatidylethanolamine (PE), unidentified lipids (L1–5) and unidentified aminolipids (AL1–4). On the basis of these data, strain lm2Tis considered to represent a novel species of the genusChryseobacterium, for which the nameChryseobacterium binzhouensesp. nov. is proposed. The type strain is lm2T(=KCTC 72529T=CCTCC AB2019126T).