Lae1 regulates expression of multiple secondary metabolite gene clusters in Fusarium verticillioides
Lae1 regulates expression of multiple secondary metabolite gene clusters in Fusarium verticillioides
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Lae1调控轮枝镰孢菌中多个次生代谢物基因簇的表达
DOI：
10.1016/j.fgb.2012.06.003
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发表时间：
2012-08-01
影响因子：
3
通讯作者：
Wiemann, Philipp
中科院分区：
文献类型：
--
作者：
Butchko, Robert A. E.;Brown, Daren W.;Wiemann, Philipp
丝状真菌黄萎镰刀菌可引起玉米病害，并能够产生伏马菌素，伏马菌素是一类有毒的次生代谢物，与人类的食道癌和神经管缺陷有关，与猪的肺水肿和马的白质脑软化症有关。伏马菌素生物合成基因的表达受广域转录因子(全局调节因子)和途径特异性转录因子Fum21的影响。LaeA是一种全球性的调节因子，它通过改变异染色质结构来影响多个次生代谢物基因簇的表达。在这里，我们使用基因缺失分析来评估黄萎病病原菌laeA同源基因的缺失对全基因组基因表达和次生代谢物产生的影响。Lae1的缺失导致负责合成次生代谢物bikverin、fumonisins、fusaric酸和fusarins的基因簇的表达减少，以及两个相应的次生代谢物未知的基因簇的表达。对次生代谢物的分析表明，与之前描述的Fujikuroi lae1突变体相比，bikverin的产量减少了。在黄萎病突变株lae1中伏马菌素的产量没有变化。黄萎病菌突变体的互补导致伏马菌素产量增加。(C)2012 Elsevier Inc.保留所有权利。
The filamentous fungus Fusarium verticillioides can cause disease of maize and is capable of producing fumonisins, a family of toxic secondary metabolites linked to esophageal cancer and neural tube defects in humans and lung edema in swine and leukoencephalomalacia in equines. The expression of fumonisin biosynthetic genes is influenced by broad-domain transcription factors (global regulators) and Fum21, a pathway-specific transcription factor. LaeA is a global regulator that in Aspergillus nidulans, affects the expression of multiple secondary metabolite gene clusters by modifying heterochromatin structure. Here, we employed gene deletion analysis to assess the effect of loss of a F. verticillioides laeA orthologue, LAE1, on genome-wide gene expression and secondary metabolite production. Loss of Lae1 resulted in reduced expression of gene clusters responsible for synthesis of the secondary metabolites bikaverin, fumonisins, fusaric acid and fusarins as well as two clusters for which the corresponding secondary metabolite is unknown. Analysis of secondary metabolites revealed that, in contrast to a previously described Fusarium fujikuroi lae1 mutant, bikaverin production is reduced. Fumonisin production is unchanged in the F. verticillioides lae1 mutant. Complementation of the F. verticillioides mutant resulted in increased fumonisin production. (C) 2012 Elsevier Inc. All rights reserved.