Osteogenic differentiation of human adipose-derived stem cells induced by osteoinductive calcium phosphate ceramics
Osteogenic differentiation of human adipose-derived stem cells induced by osteoinductive calcium phosphate ceramics
复制标题
骨诱导磷酸钙陶瓷诱导人脂肪干细胞成骨分化
DOI：
10.1002/jbm.b.31773
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发表时间：
2011-04-01
影响因子：
3.4
通讯作者：
Watari, Fumio
中科院分区：
文献类型：
--
作者：
Li, Xiaoming;Liu, Haifeng;Watari, Fumio
微观结构是磷酸钙陶瓷骨诱导的必要条件。为了研究微结构如何发挥作用并探索骨诱导的机制，我们评估了在两种化学和尺寸相同但微孔率和表面积不同的双相磷酸钙（BCP）陶瓷盘上培养的人脂肪源性干细胞的附着、增殖、碱性磷酸酶（ALP）/DNA、蛋白质/DNA和矿化。BCP-A在体内有骨诱导作用，而BCP-B无骨诱导作用。在常规培养过程中，细胞在表面积较大的BCP-A上的ALP/DNA和蛋白质/DNA均显著高于BCP-B上的细胞。预先在含50%胎牛血清（FBS）的培养基中吸附蛋白质，BCP-A的ALP/DNA和蛋白质/DNA的增量分别显著大于BCP-B的增量，表明BCP-A吸附的蛋白质量较大是关键。进一步的实验结果表明，两种预浸含rhBMP-2的培养液中细胞的ALP/DNA和蛋白/DNA均高于再浸含50%FBS的培养液中的细胞，且BCP-A的升高幅度更大。此外，在第7天，在BCP-A上发现比在BCP-B上更大的矿物质含量。结果表明，通过增加微孔和表面积，骨诱导磷酸钙陶瓷浓缩更多的蛋白质，包括骨诱导蛋白，然后刺激软组织中的诱导细胞形成诱导骨。(C)2011 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 97 B：10-19，2011.
Microstructure is indispensable for the osteoinduction of calcium phosphate ceramics. To study how microstructure takes its role and explore the mechanism of the osteoinduction, we evaluated attachment, proliferation, alkaline phosphatase (ALP)/DNA, protein/DNA, and mineralization of human adipose-derived stem cells cultured on two kinds of biphasic calcium phosphate (BCP) ceramic discs with the same chemistry and dimension, but different microporosity and surface area. BCP-A had been found osteoinductive in vivo while BCP-B was not. During the conventional culture, ALP/DNA and protein/DNA of the cell on BCP-A with larger surface area were significantly higher than those of the cells on BCP-B. With the adsorption of the proteins in culture medium with 50% fetal bovine serum (FBS) in advance, the increments of the ALP/DNA and protein/DNA for the BCP-A were found respectively significantly more than the increments of those for BCP-B, suggesting that the larger amount of protein adsorbed on the BCP-A was crucial. More results showed that ALP/DNA and protein/DNA of the cells on the two kinds of discs presoaked in culture medium having additional rhBMP-2 were found to be both higher than those of the cells on the discs resoaked in culture medium with 50% FBS, and that those values for BCP-A increased much more. Furthermore, larger mineral content was found on BCP-A than on BCP-B at day 7. The results indicated that by increasing microporosity and thus surface areas, osteoinductive calcium phosphate ceramics concentrate more proteins, including bone-inducing proteins, and thereafter stimulate inducible cells in soft tissues to form inductive bone. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 97B: 10-19, 2011.